meso-苯基-4-吡啶基取代卟啉混合物的正相高效液相色谱定量分析方法的建立

Normal Phase High Performance Liquid Chromatography Based Method to Quantity Determine Meso-phenyl-4-pyridyl Substituted Porphyrins

采用Sun FirePrep Silica正相色谱柱,选用V（二氯甲烷）∶V（正己烷）=60∶40为流动相,在检测波长为417 nm、流速为1 m L/min、柱温为25℃的条件下,建立了对meso-苯基-4-吡啶基取代卟啉混合物进行定量分析的正相高效液相色谱方法。在该定量分析方法中,6种卟啉的标准曲线线性相关系数均大于0.999 3,相对标准偏差均小于1.28%,加标回收率在96.4%~103.9%之间。实验结果表明,该定量分析方法可用于meso-苯基-4-吡啶基取代卟啉混合物的同时测定。

A method for the quantitative analysis of meso-phenyl-4-pyridyl substituted porphyrins was established by normal phase high performance liquid chromatography（ NP-HPLC）.NP-HPLC was carried out on a Sun FirePrep Silica normal column,using V（ dichloromethane） ∶ V（ n-hexane） = 60 ∶40 as the mobile phase and the detection was performed by PAD detector at 417 nm,of which flow rate was 1. 0 m L/min and the column temperature was 25 ℃.In the quantitative analysis method,the linear correlation coefficients of all the six porphyrins were more than 0. 999 3,the relative standard deviations were less than 1. 28%,and the recoveries were between 96. 4% and 103. 9%.This method can be used for the simultaneous determination of meso-phenyl-4-pyridyl substituted porphyrins.