脑梗死患者肠道微生态结构的变化和复杂度分析

The structural and diversity analysis of intestinal microecology on cerebral infarction patients

目的通过测序技术检测脑梗死患者肠道微生态结构的变化并进行复杂度分析。方法选取健康志愿者(Norm组)和脑梗死患者(CI组)各10例取其粪便标本,应用16s测序技术检测其肠道微生态的结构变化,通过Unweighted Unifrac距离衡量Norm组和CI组所有样本间的相异系数。结果 CI组与Norm组相比肠道菌群在纲水平的结构差异显著不同。同Norm组相比,CI组梭菌纲、变形杆菌纲、柔膜菌纲显著增加,拟杆菌纲、疣微菌纲、放线菌纲、α-变形菌纲、芽孢杆菌纲、鞘脂杆菌纲、β-变形菌纲显著降低,差异均有统计学意义(P<0.05)。Norm组和CI组肠道菌群的复杂度差异较大。结论脑梗死患者存在肠道微生态紊乱的现象,与健康人群存在显著性差异,因此肠道菌群可作为脑梗死的潜在预测手段和治疗靶点。

Objective To test the structural and diversity analysis of intestinal microecology on cerebral infarction patients by using the sequencing technology. Methods Both 10 fecal samples of healthy people（Norm group） and cerebral infarction patients（CI group） was selected to detect the intestinal tract structural changes in the microecology by using 16s sequencing technology. The dissimilarity coefficient between the two groups was checked by Unweighted Unifrac. Results There were significant differences in the structural differences between the CI group and the Norm group at the class level. Compared with Norm group, the Clostridia, Gammaproteobacteria and Mollicutes were increased, Bacteroidia,Verrucomicrobiae, Actinobacteria, Alphaproteobacteria, Bacilli, Sphingobacteriia and Betaproteobacteria were decreased in CI group,the differences were statistically significant（P0.05）. There was a significant diversity between Norm group and CI group. Conclusion The intestinal microecological of CI patients was disorganized,whose diversity was significant difference compared with healthy people.The intestinal microecological may be the potential prediction methods and therapeutic targets for CI patients.