唾液酸化人乳寡糖的分离及串联质谱结构序列分析

Separation and Structural Sequence Analysis of Sialylated HMOs via Tandem Mass Spectrometry

基于不同唾液酸化寡糖之间的极性、空间结构及电荷密度的差异性,采用半制备型多孔性石墨化碳(PGC)色谱分离制备了高纯度唾液酸化人乳寡糖单体,并使用离线的电喷雾-串联质谱技术(ESI-MSn)确定8种寡糖单体的序列结构分别为6'-SL,3'-SL,LSTb,LSTc,F-LSTc,F-LSTb,DSLNT与FS-LNn H.本文结果为进一步从事唾液酸化人乳寡糖的结构与活性研究提供了参考依据.

Sialylated human milk oligosaccharides（ SHMOs）, important components of human milk oligosaccharides,play a key role in the growth and development of the newborn. Herein,based on the difference of polarity,spatial structure and charge density of sialylated oligosaccharides,a semi-preparative porous graphitized carbon（ PGC） chromatography was employed to separate SHMOs in high purity. An off-line electrospray ionization tandem mass spectrometry（ ESI-MSn） was performed to identify the sequential structures of eight oligosaccharides including 6＇-SL,3＇-SL,LSTb,LSTc,F-LSTc,F-LSTb,DSLNT and FS-LNn H. This study provides a useful reference for the further structure-activity analysis and application development of SHMOs.