Stathmin基因在卵巢癌组织中的表达水平及其与紫杉醇联合顺铂敏感性的研究

Expression level of Stathmin gene in ovarian cancer tissues and its relationship with the sensitivity of paclitaxel and cisplatin

目的观察Stathmin基因在卵巢癌组织中的表达水平及其与紫杉醇联合顺铂化疗敏感性关系。方法选取30例进行根治性切除术的原发性卵巢癌患者癌组织(试验组)和癌旁正常组织(对照组)作为研究对象。用免疫组化法检测Stathmin基因的表达水平,用甲基环戊二烯基三羰基锰(MMT)检测卵巢癌组织对0.30,3.00,30.00,300.00 mg·L^(-1)紫杉醇联合顺铂的敏感性,分析卵巢癌组织中Stathmin基因表达水平和紫杉醇联合顺铂化疗敏感性关系。结果试验组和对照组的Stathmin基因阳性率分别为53.33%和10.00%,差异有统计学意义(P<0.05)。0.30 mg·L^(-1)组的高度敏感、中度敏感和不敏感构成比为分别为30.00%,50.00%和20.00%,3.00 mg·L^(-1)组的高度敏感、中度敏感和不敏感构成比为分别为43.33%,40.00%和16.67%,30.00 mg·L^(-1)组的高度敏感、中度敏感和不敏感构成比为56.67%,26.67%和16.67%,300.00 mg·L^(-1)组的高度敏感、中度敏感和不敏感构成比为20.00%,16.67%和63.33%。300.00mg·L^(-1)组和30.00 mg·L^(-1)组的高度敏感和不敏感构成比比较,差异均有统计学意义(均P<0.05)。300.00 mg·L^(-1)组和0.30 mg·L^(-1)组的中度敏感和不敏感构成比比较,差异均有统计学意义(均P<0.05)。30.00 mg·L^(-1)组和0.30mg·L^(-1)组的高度敏感构成比比较,差异有统计学意义(P<0.05)。以30.00mg·L^(-1)紫杉醇联合顺铂处理卵巢癌细胞,高度敏感组、中度敏感组和不敏感组Stathmin基因阳性率分别为29.41%,62.50%和80.00%,差异均有统计学意义(均P<0.05)。结论 Stathmin基因在卵巢癌组织中表达水平显著高于癌旁正常组织,且与紫杉醇联合顺铂敏感性存在一定关联。

Objective To explore and study the expression level of Stathmin gene in ovarian cancer tissues and its relationship with paclitaxel and cisplatin chemotherapy. Methods The tumor tissues and adjacent normal tissues of 30 cases of patients with primary ovarian cancer were selected as the research group and the control group. The expression level of Stathmin protein was detected by Immunohistochemistry, and sensitivi- ty of ovarian tumor tissues to O, O. 30, 3.00,30. 00,300. 00 mg·L-1 paelitaxel combined with cisplatin was analyzed by Methyl -cyelopenta- dienyl- Manganese - Triearbonyl （MMT）. The relationship between Stathmin expression in ovarian cancer tissues and the sensitivity of pacli- taxel and eisplatin chemotherapy were analyzed. Resulls The positiverates of Stathmin gene in the treatment group and control group were respectively 53.33% and 10. 00% , and the diffe- rence was significantly different （ P 〈 0. 05 ）. The rates of hypersensitivity, medium sensitivity and insensitivity were 30.00% , 50. 00% and 20.00%in the 0.30 mg·L-1group, 43.33% ,40. 00% and 16.67% in the 3.00 mg·L-1 group, 56.67% ,26.67% and 16.67% in the 30.00 mg·L-1 group, 20.00%,16.67% ,63.33% in the 300.00 mg·L-1group. There were significant differences in hypersensitivity and insensitivity between the 30. 00 mg·L-1group and the 300. 00 mg·L-1 group （ all P 〈 0. 05 ）. There were significant differences in medium sensitivity and in- sensitivity between the 300.00mg·L-1 group and the 0.30 mg·L-1group （ all P 〈 0. 05 ）. There was significant difference in hypersensitivity between the 30. 00 mg·L-1 group and the 0. 30 mg·L-1 group （ P 〈 0. 05 ）. After treat- ment with 300. 00 mg·L-1 paclitaxel combined with cisplatin, the positive rates of Stathmin gene were 29.41% , 62. 50% and 80. 00% , and the differences were significantly different （ all P 〈 0. 05 ）. Conclusion The expression level of Stathmin in ovarian cancer tissues is significantly higher than that in adjacent normal tissues, whichis related to the sensitivity to paclitaxel and cisplatin.