粪便标本小肠结肠炎耶尔森菌筛检方法的比较

Comparison of screening methods on Yersinia enterocolitica in fecal specimen

目的比较分离培养法、反转录-聚合酶链反应法对腹泻粪便中小肠结肠炎耶尔森菌的检测情况。方法在2016年6月至2017年6月临床收治的腹泻患者中选取368例,均使用分离培养法、反转录-聚合酶链反应法对其粪便中的小肠结肠炎耶尔森菌展开检验,对比分析两种方法的检验结果。结果反转录-聚合酶链反应法阳性率为77.17%,比分离培养法的51.09%高,差异有统计学意义(P<0.05);反转录-聚合酶链反应法致病菌株检出率为83.10%,高于分离培养法的59.57%,差异有统计学意义(P<0.05)。结论对腹泻患者粪便标本中的小肠结肠炎耶尔森菌检测时,相较于分离培养法,反转录-聚合酶链反应法阳性检出率更高,可使小肠结肠炎耶尔森菌性腹泻诊断准确率提升。

Objective To compare the application of isolation culture method and reverse transcription-poly- merase chain reaction method in the test of enterocolitis in diarrhea feces.. Methods 368 cases diarrhea pa tients were collected from June 2016 to June 2017. Reverse transcription-polymerase chain reaction method and isolation culture were used to test Yersinia enterocolitica. Results The positive rate of reverse tran scription-polymerase chain reaction was 77. 17%, 51.09% higher than the separation culture method, with statistical significance （P〈0.05）; reverse transcription-polymerase chain reaction pathogenic bacteria detec- tion rate was 83.10%, higher than the culture method of the 59.57%, there was statistical significance （p d0.05） . Conclusion Compared with the isolation culture method, the positive rate of reverse transcrip- tion-polymerase chain reaction is higher in the detection of Y. enterocolitica screening in stool specimens of diarrhea patients, which can improve the diagnostic accuracy of Yersinia enterocolitica diarrhea.