急性髓系白血病患者14-3-3zeta基因表达水平及其临床意义

Expression levels and clinical significance of 14-3-3zeta gene in patients with acute myeloid leukemia

目的:通过检测14-3-3zeta基因在成人急性髓系白血病(AML)患者中的mRNA转录水平,探讨其与AML患者临床特征和治疗反应的相关性。方法:采用实时定量PCR检测12例正常人和63例AML患者的14-3-3zeta mRNA转录表达量,用Western blot检测部分患者14-3-3zeta蛋白的表达,分析其与临床特征及治疗反应的相关性。结果:63例AML患者细胞内14-3-3zeta转录水平显著高于正常人(P<0.05),其中20例完成了1个疗程以上的治疗,14-3-3zeta转录水平在治疗达到完全缓解后表达下降,在巩固期持续低表达,2例复发时转录和蛋白水平均再次升高。14-3-3zeta转录表达水平女性高于男性,但与AML患者的年龄、白细胞计数及WHO分类无明显相关性。结论:AML患者14-3-3zeta mRNA转录水平高可能与AML发生有关,与疗效和转归存在负相关,是否预后相关不良因素仍需要进一步扩大样本研究。

Objective：To investigate the expression levels of 14-3-3 zeta gene in patients with acute myeloid leukemia（AML）,and to explore its correlation with the clinical characteristics and therapeutic reaction.Method：The mRNA levels of 14-3-3 zeta from 63 AML patients and 12 healthy controls were detected by real-time fluorescence quantitative polymerase chain reaction（RQ-PCR）.Western blot was performed to investigate the expression of 14-3-3 zeta proteins.The correlation of 14-3-3 zeta mRNA level with clinical characteristics,complete remission and survival time was analyzed.Result：The mRNA expression levels of 14-3-3 zeta gene in 63 AML patients were significantly increased compared with healthy controls（P0.05）.The mRNA expression levels of the patients with complete remission（CR）decreased and kept lower levels.But the mRNA and protein expression levels of 14-3-3 zeta gene increased again when the patients relapsed.The mRNA expression levels of 14-3-3 zeta gene in female patients were higher than those in male patients and it did not relate with age,white cell count and subtype,respectively.Conclusion：The mRNA levels of 14-3-3 zeta gene are significantly related with pathogenesis and therapeutic reaction,which needs large sample study to verify.