牡丹(Paeonia Sect. Moutan)生物技术育种研究进展

Advances in Research on Biotechnology Breeding of Tree Peony(Paeonia suffruticosa)

生物技术育种是牡丹品种改良和种质创新的重要手段。本研究从组织培养、遗传多样性、DNA指纹图谱的构建、功能基因、遗传图谱构建等方面总结了牡丹生物技术育种研究进展,并根据存在的问题对未来研究的重点方法、领域及发展的方向进行了探讨。牡丹组织培养研究中,生根依旧是难点,分生结节将成热点。牡丹遗传多样性的研究以及构建指纹图谱和遗传图谱,分子标记开发和应用是重点,全面而准确的DNA指纹图谱的构建以及重要性状QTLs的定位是研究热点。牡丹功能基因研究的重点方向则是基因功能验证及重要性状的分子调节机制。本研究旨在推动牡丹生物技术育种的发展,为相关技术创新、分子标记辅助选择育种及转基因育种提供参考。

Biotechnology breeding is an important means in improving tree peony varieties and contriving new germplasm. This paper summarized the advances in research on biotechnology breeding for tree peony, including tissue culture, genetic diversity, DNA fingerprint database construction, functional genes, genetic map construction, and so on. According to some existing problems, this paper also discussed the major methods, fields and development directions in the future studies. For tree peony tissue culture, rooting is still difficult and meristematic nodule will become hotspot. The development and application of molecular markers are recommended in research on genetic diversity and construction of DNA fingerprint database and genetic map for tree peony. Meanwhile, comprehensive and accurate DNA fingerprint database construction and QTLs location for phenotypic traits in tree peony are considered as the key fields. The major directions of tree peony functional genes are gene function verification and molecular regulation mechanism of key traits. The paper aimed at promoting the development of biotechnology breeding in tree peony, so as to provide a reference for its related technical innovations, molecular assisted selection breeding and transgenic breeding.