薰衣草产酚酸内生真菌的分离及产物初步鉴定

Isolation of Endophytic Fungi of Phenolic Acids in Lavender and the Identification of Its Products

利用人工种植的薰衣草及无锡本地采集的相同品种薰衣草,对其各组织内生真菌进行分离、纯化。分别分离得到18和23株内生真菌。对所筛选出的菌株进行固态发酵,并对其发酵培养产物进行提取。通过化学反应的方法从野外采集薰衣草内生真菌中筛选出一株产酚酸的菌株,并通过分子生物学方法对其进行初步鉴定,并对其固态发酵提取物进行初步研究。研究结果表明了薰衣草内生真菌的多样性,为通过微生物固态发酵产酚酸类物质提供理论和实验基础。

In this study,the endophyte fungi of lawender was isolated and identified.18 and 23 endophytic fungi were isolated from planting lavender and wild lavender. The strains were cultured by solid-state fermentation and to extract the fermentation culture products. Wild lavender extracted a strain endophytic fungi of phenolic acids by using the method of chemical reaction. And the strains were identified by the method of molecular biology. Solid-state fermentation extract was identified primarily. The diversity of endophytic fungi of lavender is reflected by the results of the study and the solid fermentation by microorganisms to produce phenolic acid material provided theoretical and experimental basis.