LP-17多肽对牙龈卟啉单胞菌脂多糖致牙周炎进展的作用

Effect of LP-17 on Porphyromonasgingivalis Lipopolysaccharide Caused Periodontitis

目的:探究髓系细胞触发受体-1(TREM-1)的抑制剂LP-17多肽对牙龈卟啉单胞菌脂多糖(P.gingivalis-LPS)诱导的小鼠牙周炎进展的作用。方法:在C57BL/6小鼠上颌第二磨牙龈沟内隔日注射P.gingivalisLPS(10μg),构建实验性牙周炎模型。每日给予腹腔注射LP-17(10 mg/kg),对照组注射PBS。14d后处死小鼠,收集牙槽骨,micro-CT测量牙槽骨的吸收量,HE染色检测牙周组织的病理学表现,TRAP染色检测牙周组织中破骨细胞的数目,免疫组化检测牙周组织中TREM-1的表达情况。结果:龈沟内注射P.gingivalis-LPS成功诱导了小鼠的实验性牙周炎。LP-17多肽能够减轻牙周组织的炎症细胞浸润和胶原纤维变性程度,抑制牙周组织中TREM-1的表达,减少破骨细胞数量和牙槽骨吸收量。结论:LP-17多肽可以减轻P.gingivalis-LPS诱导的牙周炎症反应,延缓牙周炎进展。

Objective：To explore the effect of LP-17,an inhibitor of triggering receptors expressed on myeloid cells-1（TREM-1）on porphyromonasgingivalis lipopolysaccharide（P.gingivalis-LPS）induced periodontitis in mice.Methods：P.gingivalis-LPS was injected into maxillary second molar sulcus of C57 BL/6 mice to construct experimental periodontitis model.Mice were treated with LP-17 peptides by intraperitoneal injection every day,and PBS was set as the control.After 14 days,all mice were sacrificed.Micro-CT was used to measure the alveolar bone absorption.HE staining was used to assess pathological alteration and TRAP staining was used to detect osteoclasts in periodontium.In addition,the expression of TREM-1 was determined by immunohistochemistry.Results：P.gingivalis-LPS induced experimental periodontitis in mice.LP-17 reduced the degree of collagen fibrosis and inflammatory cell infiltration.Additionally,LP-17 inhibited the expression of TREM-1 in periodontal tissue.Furthermore,LP-17 treatment notably decreased the number of osteoclasts and alveolar bone absorption.Conclusion：LP-17 peptides alleviated the inflammatory response in periodontal tissue,thus arrested the progress of periodontitis.