实时直接分析质谱法快速鉴别白芷药材

Rapid Identification of Angelicae Dahuricae Radix by DART-MS

目的：建立一种白芷药材的快速鉴别方法。方法：采用实时直接分析离子源（DART）结合离子阱质谱快速鉴别白芷药材,高纯氦气为解离气,载气温度300℃,格栅电压＋350 V。定性分析采用比对特征离子和欧前胡素、异欧前胡素二级质谱进行。结果：该方法样品制备简单,可以在几秒内得到样品质谱图,实现样品鉴别。通过对不同批次白芷药材定性鉴别证明了该方法的可行性。结论：本方法可用于白芷药材的快速定性鉴别。

Objective： To establish a rapid method for identification of Angelicae Dahuricae Radix. Methods： Direct analysis in real time ionization source（ DART） coupled with ion trap mass spectrometry was employed to rapidly identify Angelicae Dahuricae Radix. The high-purity helium（ purity ≥99. 999%） was used as the ionising medium. The ionisation gas temperature was set to 300 ℃ and the grid electrode voltage of DART was set to ＋ 350 V. The characteristic ion and DART-MS/MS spectra of Imperatorin and Isoimperatorin were used to identify sample materials. Results： With minimal sample pretreatment,mass spectra of Angelicae Dahuricae Radix samples were obtained within seconds. Successful authentication was achieved by analyzing the same materials of different origins. Conclusion： This method is suitable for the rapid analysis of Angelicae Dahuricae Radix.