miR-1/133基因簇的进化及其调控网络

The evolution analysis and regulation network of miR-1/133 gene cluster

miR-1/133基因簇在心肌和骨骼肌中特异性表达,对心脏以及骨骼肌的发育及生理病理具有重要作用.该研究采用生物信息学方法,研究了miR-1/133基因簇的进化特征,预测了其靶基因及其调控的生物学过程.利用miRBase数据库对23种不同物种间miR-1/133基因簇的序列、组织方式进行了比较.采用最大似然法构建miR-1/133基因簇的系统进化树,显示该基因簇的进化与物种进化关系有一定差异.利用在线数据库对miR-1/133基因簇上游转录因子及下游靶基因进行预测,并对其进行综合分析,绘制出该基因簇的网络调控图,表明miR-1/133基因簇参与了骨骼肌与心肌发育、胰岛素受体信号通路、经典Wnt信号通路、p53信号通路等体内重要生理过程,为进一步阐明miR-1/133基因簇的生物学功能提供了理论依据.

To investigate the molecular evolution and biological function of miR-1/133 gene cluster,we searched comment information and analyzed the structure and sequence characteristics of miR-1/133 gene cluster by using miRBase database.The phylogenetic trees were constructed by Maximum Likelihood method.The upstream transcription factors and target genes of miR-1/133 gene cluster were studied by integrating compressive information from different online databases.The phylogenetic trees analysis showed that there is some differences between molecular evolution of miR-1/133 gene cluster and the evolution of animals.The miR-1/133 gene cluster is highly conserved and stably exists in Bilaterians.The predicted target genes were involved in some important signaling pathways,including Skeletal muscle and myocardial development,insulin receptor signaling pathway,Wnt signaling pathway,P53 signaling pathway.Bioinformatic analysis of molecular evolution and the regulation network of the miR-1/133 gene cluster potentially provide a theoretical basis for deeper insights into its biological function.