响应面优化酶协同超声提取四角菱角壳多酚工艺

Optimization of Enzymatic Ultrasonic-Assisted Extraction of Polyphenols from Pericarps of Trapa natans L. Var. incisa Makino by Response Surface Methodology

目的：优化四角菱角壳中多酚的酶协同超声提取工艺。方法：采用酶协同超声提取法,在单因素试验基础上,以多酚提取量为应变量,以纤维素酶浓度、超声提取时间、提取温度为自变量,采用响应面设计方法优化四角菱角壳中多酚的提取工艺。结果：最佳提取工艺为用pH 4.5的1.86%纤维素酶溶液30 m L·g-1预处理30 min后,在53℃下超声提取26 min,在此条件下多酚提取量为161.3 mg·g-1,与模型预测值（158.14 mg·g-1）相符,相对标准偏差2.00%。与乙醇超声提取和回流提取相比,酶协同超声提取多酚提取量显著提高。结论：响应面法优化四角菱角壳多酚的酶协同超声提取工艺方法简单、提取效率高、可预测性良好,值得推广使用。

Objective： To optimize theenzymatic ultrasonic-assisted extraction of polyphenols from the pericarps of Trapa natans L. Var. incisa Makino. Methods： Based on the single factor results,the independent variables such as cellulase concentration,ultrasonic time and extraction temperature were optimized by response surface methodology（RSM） with polyphenols yields as dependent variable. Results： The optimal conditions obtained by RSM with enzymatic ultrasonic-assisted extraction were as follows：water-material ratio of 30 m L·g-1,pH = 4. 5,1. 86% cellulase pretreated 30 min,extraction temperature 53 ℃,and extraction time 26 min. Under this optimal conditions,the experimental polyphenols yields were161. 3 mg·g-1,which was well agreed with model predictions of 158. 14 mg·g-1with RSD 2. 00%. Conclusion： The extraction process of polyphenols from Trapa natans L.Var. incisa Makino by enzymatic ultrasound-assisted was stable and efficient with high precision and good predictability.