乳腺癌组织中lncRNA MALAT1的表达及临床意义

Expression and Clinical Significance of Long Non-coding RNA MALAT1 in Breast Cancer

目的探讨长链非编码RNA MALAT1在乳腺癌组织中的表达及临床意义。方法采用实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,q RT-PCR)方法检测64例乳腺癌患者癌组织标本及其对应的癌旁组织标本中MALAT1的表达情况,分析其与乳腺癌的临床病理特征关系以及诊断价值。结果 MALAT1在乳腺癌组织中的表达明显高于癌旁组织(P<0.05),且癌组织中MALAT1的表达水平与ER、PR表达情况以及淋巴结转移明显相关明显相关(P<0.05);ROC曲线分析发现AUC为0.706。结论组织中高表达的MALAT1可作为乳腺癌诊断的一个潜在的新型生物学标志物。

Objective To investigate the expression and clinical significance of long non-coding RNA （lncRNA） metastasis-associated lung adenocarcinoma transcript 1（MALAT1） in breast cancer. Methods MALAT1 levels were measured in breast cancer tissue samples and corresponding adjacent tissues from 64 breast cancer patients using quantitative real-time PCR. The correlation between MALAT1 levels and clinical pathological characteristics of breast cancer was analyzed.A receiver operating characteristic （ROC） curve was constructed to determine the diagnostic value of MALAT1. Results The expression levels of MALAT1 in cancer tissues were significantly higher than those in adjacent tissues （P〈0.05）. The expression levels of MALAT1 in breast cancer patients correlated with expression of ER, PR and lymph node metastasis （P〈0.05）; the area under the ROC curve was 0.706. Conclusion Plasma lncRNAMALAT1 may be involved in carcinogenesis and prove as a potential biomarker for the diagnosis of breast cancer.