羊接种布鲁氏菌S2菌苗抗体消长规律研究

Growth and Decline Regularity of Antibodies in Sheep Inoculated with Brucellosis S2

本研究探讨不同途径给羊接种布鲁氏菌S2菌苗后抗体消长规律,以及用4种方法检测菌苗抗体结果的符合程度。选取44只在相同饲养条件下的北京土种绵羊进行单次免疫,分为注射组20只,50×108CFU/只;灌喂组24只,100×108CFU/只。在免疫后的第3、7、13、17、21、25周采血分离血清,用虎红平板凝集试验(RBPT),试管凝集试验(SAT),微量凝集试验(MAT)和酶联免疫吸附试验(ELISA)方法,对这44份血清抗体进行检测并分析。结果显示:注射组在第7周仍能保持一定的抗体水平,直至第25周仍能检测到抗体,而灌喂组在第13周阳性率已为0。3种检测方法与ELISA比较κ值分别为:κRBPT vs ELISA=0.805,κSAT vs ELISA=0.760,κMAT vs ELISA=0.899。肌肉注射比灌喂接种方式有更高的抗体阳性率,且该抗体能维持半年。微量凝集试验与酶联免疫吸附试验抗体检测结果的一致性最高。

We aim to discuss the growth and decline regularity of antibodies in the sheep inoculated with Brucella S2 by different approaches and to analyze fitting degree of the antibody results detected by different methods. We selected 44 Beijing native sheep under the same feeding conditions to perform single immunization. Twenty sheep were immunized by injection, with 5×10~8 CFU per sheep, the other twenty-foursheep were immunized by feeding, with 100 ×108 CFU per sheep. On the 3^（rd）, 7^（th）, 13^（th）, 17^（th）, 21^（st） and 25^（th） week,blood was sampled to separate serum. Then the 44 serum antibody samples were tested and analyzed with rosebengal plane test（RBPT）, standard-tube agglutination test（SAT）, micro-agglutination test（MAT） and enzymelinked immunosorbent assay（ELISA）. The results showed that the serum of injection group maintained acertain antibody level on the 7 thweek and the antibody could be detected till the 25 thweek. However, thepositive rate of feeding group was 0% on the 13 thweek. The Kappa values of RBPT, SAT and MAT compared with ELISA were 0.805, 0.760 and 0.899, respectively. The positive rate of antibodies in the injection group was higher than that in the feeding group, and the antibodies could be maintained for half a year. The consistency of the antibody test result was the highest between MAT and ELISA.