响应面法优化超临界二氧化碳萃取蓝圆鲹鱼油工艺研究

Optimization of supercritical carbondioxide extraction of Decapterus maruadsi fish oil by response surface methodology

利用超临界二氧化碳萃取技术提取蓝圆鲹鱼油,采用单因素实验考察萃取温度、萃取压力及萃取时间对蓝圆鲹鱼油得率的影响,并采用响应面优化Box-Behnken设计优化萃取工艺。结果表明:最佳萃取工艺条件为萃取温度49℃、萃取时间140 min、萃取压力20 MPa,在此条件下蓝圆鲹鱼油得率为18.45%,提取率达88.91%。蓝圆鲹鱼油经气相色谱-质谱法(GC-MS)分析鉴定出17种脂肪酸,不饱和脂肪酸含量为55.70%,其中二十碳五烯酸(EPA)与二十二碳六烯酸(DHA)总含量为16.64%。超临界二氧化碳萃取技术应用于蓝圆鲹鱼油的提取具有一定的理论价值和经济效益。

The Decapterus maruadsi fish oil was extracted by supercritical carbon dioxide extraction technology, and the effects of extraction temperature, extraction pressure and extraction time on the yield of Decapterus maruadsi fish oil were investigated by single factor experiment. And the optimization of extraction process was carried out by Box-Behnken design and response surface methodology. The results showed that the optimal extraction conditions were obtained as follows： extraction temperature 49？℃, extraction time 140 min and extraction pressure 20 MPa. Under the optimal conditions, the yield ofDecapterus maruadsi fish oil was 18.45% and the extraction rate was 88.91%. Seventeen kinds of fatty acids were identified from Decapterus maruadsi fish oil by gas chromatography-mass spectrometry （GC-MS）, and the content of unsaturated fatty acids was 55.70%, in which the total content of EPA and DHA was 1664%. The application of supercritical carbon dioxide extraction technology in Decapterus maruadsi fish oil extraction had certain theoretical value and economic benefits.