沙棘油脂肪酸、sn-2位脂肪酸及甘三酯的测定与分析

Determination and analysis of fatty acid,sn-2 fatty acid and triacylglycerol in sea buckthorn oil

采用超临界CO_2萃取得到沙棘籽油和沙棘全果油,并采用气相色谱和超高效液相色谱-飞行时间质谱对其脂肪酸、sn-2位脂肪酸及甘三酯组成进行测定与分析。结果表明:沙棘籽油中亚油酸、亚麻酸和油酸的含量较多,分别为38.71%、25.66%和20.68%,这3种脂肪酸也是其主要的sn-2位脂肪酸,含量分别为46.46%、25.49%和23.02%;LOL(13.65%)、OLO(12.06%)和LLn O(11.23%)是沙棘籽油的主要甘三酯类型;沙棘全果油中棕榈油酸(36.86%)、棕榈酸(27.27%)和油酸(18.00%)的含量较高,主要的sn-2位脂肪酸为棕榈油酸(45.57%)和油酸(32.06%);沙棘全果油的主要甘三酯为POPo(14.38%)、PoOPo(13.77%)和OPO(12.06%)。

Two kinds of sea buckthorn oils were individually extracted from the sea buckthorn pulp and seed by supercritical CO2 technique. Their fatty acid, sn-2 fatty acid and triacylglycerol compositions were determined by GC and UPLC-Q-TOF/MS, respectively. The results demonstrated that linoleic acid （38.71%）, linolenic acid （25.66%）and oleic acid （20.68%）were the major fatty acids in the seed oil. Meanwhile, their contents in sn-2 fatty acids were also dominant, reaching 46.46%, 2549% and 23.02%, respectively. The main triacylglycerols in the seed oil were LOL （13.65%）, OLO （12.06%）and LLnO （11.23%）. Palmitoleic acid （36.86%）, palmitic acid （27.27%）and oleic acid （18.00%）were the predominant fatty acids in the pulp oil. Palmitoleic acid （45.57%）and oleic acid （32.06%） were the main sn-2 fatty acids, moreover, POPo （14.38%）, PoOPo （13.77%）and OPO （12.06%）were the predominant triacylglycerols in the pulp oil.