高效液相色谱法测定黄七胶囊中人参皂苷Rg_1、Rb_1及三七皂苷R_1的含量

Content Determination of Ginsenoside Rg_1,Rb_1 and Notoginsenoside R_1 in Huang Qi Capsules by HPLC

目的:优选流动相参数,建立高效液相色谱法(HPLC)测定复方制剂黄七胶囊中人参皂苷Rg1、Rb_1及三七皂苷R_1含量的方法。方法:以十八烷基硅烷键合硅胶为填充剂,以乙腈为流动相A,以水为流动相B,考察最佳梯度洗脱参数。结果:最佳梯度为0~30 min,乙腈19→40,水81→60。在此条件下,人参皂苷Rg_1进样量在0.849 6~7.646 4μg,Rb_1在0.853 8~8.853 8μg,三七皂苷R1在0.202 0~2.020 0μg范围内皆与峰面积呈良好的线性关系。结论:该流动相梯度洗脱比例提高了峰面积和理论塔板数,并节省了时间。

Objective： To choose optimal flow phase parameters and establish a content determining of ginsenoside Rg1,Rb and notoginsenoside R1 in Huang Qi Capsules by HPLC. Methods： The octadecyl silane chemically bonded silica was used as filler,acetonitrile as mobile phase A and water as mobile phase B. Results： The optimum gradient： 0 - 30 min,acetonitrile： 19 →40,and water： 81 →60. On this condition,the sample size of ginsenoside Rg1： 0. 8496 - 7. 6464 μg,Rb1： 0. 8538 - 8. 8538 μg,and notoginsenoside R1： 0. 2020 - 2. 0200 μg,were all in good liner relation with peak area. Conclusion： The mobile phase gradient elution scale improved peak area and number of theoretical plates,and saves time.