玉屏风散对煤烟相关可吸入颗粒物致肺损伤模型小鼠免疫功能的干预作用

Effect of Yupingfeng San on Immune Function of Lung Injury Mice Induced by Smoked Soot-related Respirable Particulate

目的：探讨玉屏风散对煤烟相关可吸入颗粒物小鼠肺损伤模型免疫功能的干预作用。方法：将实验小鼠置于特制染毒箱中,使煤炭燃烧产生的烟雾通入该箱,箱内PM2.5浓度维持在700-900μg·m^-3,建立吸入颗粒小鼠肺损伤模型。以玉屏风散中药水煎剂对染毒模型分别进行预防用药、预防＋治疗用药、治疗用药3种方式灌胃给药,检测肺泡灌洗液中尘细胞比例,免疫组化法检测肺组织中CD68＋巨噬细胞数量,流式细胞技术检测血CD4＋和CD8＋细胞及其比例,酶联免疫吸附法（ELISA）检测血清、肺组织匀浆免疫球蛋白E（Ig E）,Ig G浓度。结果：模型组小鼠肺泡灌洗液中尘细胞和CD68＋巨噬细胞数量显著增多,血CD4＋,CD8＋均降低,血清Ig E水平升高,Ig G水平降低,肺组织匀浆中Ig E无明显变化,Ig G水平显著降低。药物干预后,血液中CD4＋,CD8＋细胞数量升高,但二者比例各组间无统计学差异。尘细胞数量和CD68＋巨噬细胞数量明显降低,以预防＋治疗组最为明显。血清中Ig E含量以预防组最低,血清和肺组织匀浆Ig G则预防＋治疗组最高。结论：玉屏风散预防应用可提高小鼠的细胞及体液免疫功能,且在染毒环境中保持,但在已染毒后的治疗作用中效果远不如其提前应用疗效更佳。

Objective： To investigate the effect of Yupingfeng San on the immune function of mice models with lung injury induced by smoked soot-related respirable particulate. Method： The experimental mice were placed in a special exposure box,and the smoke from the combustion of coal was introduced into the box. The PM2. 5 concentration in the box was maintained at 700-900 μg·m^-3 to establish the model of lung injury. The Yupingfeng San decoction was given to the models by intragastric administration in 3 different ways： prevention,prevention ＋ treatment,and treatment. Then the proportion of dust cells in the alveolar lavage fluid was detected;the count of CD68＋macrophages in the lung tissues was detected by immunocytochemistry; CD4＋and CD8＋cells counts and their ratio were detected by flow cytometry; Ig E and Ig G concentrations in serum and lung tissues were detected by enzyme-linked immunosorbent assay（ELISA）. Result： The dust cells count and CD68＋macrophages count in alveolar lavage fluid of model mice were increased significantly; serum CD4＋,CD8＋were decreased; the Ig E level in serum was increased and the Ig G level in serum was decreased; Ig E level in the lung homogenate had no obvious change,while the Ig G level in lung homogenate was decreased significantly in model group. After drug intervention,the count of CD4＋and CD8＋cells in the blood was increased,but there was no statistical difference between the two groups. The count of dust cells and the count of CD68＋macrophages were decreased significantly,most obvious in the prevention ＋ treatment group. The Ig E level in serum was lowest in the prevention group,and the Ig G level in serum and lung homogenate was highest in the prevention ＋ treatment group. Conclusion：Yupingfeng San can improve the cellular and humoral immune function of mice,and the effect can be maintained in toxic environment; however the effect in the toxic environment is far less than the earlier application effect.