科尔沁地区感染HPV人群亚型分析

Clinical Analysis of HPV Infection Subtypes in the Khochin District of Inner Mongolia

目的:探讨科尔沁地区人群HPV感染的亚型差异.方法:以专用的宫颈细胞采集器进行采样,利用PCR体外扩增和DNA反向点杂交相结合的DNA芯片技术,测HPV23种亚型,其中高危型HPV共17种分别是16、18、31、33、35、39、45、51、52、53、56、58、59、66、68、73、82,低危型HPV共6种分别是6、11、42、43、81、83.检查者为门诊、住院及体检的科尔沁地区妇女,共5336人.结果:在5336例分型检测结果中,HPV阳性例数2534例,感染率为47.49%.在HPV阳性的病人中排在前三位的HPV亚型,低危型依次为HPV6、43、81;高危型依次为HPV16、18、33.结论:在科尔沁地区HPV感染以16型和6型为主,可为该地区的HPV感染的合理化预防和治疗提供依据.

Objectives： To investigate the subtype difference of HPV infections in the Khorchin district. Methods：Sampling was carried out with a special cervical cell collector, using DNA chip technology with PCR amplification combined with DNA reverse dot hybridization to measure 23 subtypes of HPV among samples： 17 kinds of high-risk subtypes： HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73 and 82;6 kinds of low-risk types： HPV6,11, 42, 43, 81 and 83. In total 5336 study subjects were outpatient, inpatient and physical examination women from the Khorchin region. Results： From the 5336 cases of typing detection samples, 2534 were HPV positive, and the infection rate was 47.49%. The top three subtypes among the HPV positive patients for low-risk type were HPV6, 43 and 81 and for high-risk type, HPV16, 18 and 33. Conclusion： The HPV infection in the Khorchin district was dominated by subtypes 6 and 16, which provides a basis for reasonable prevention and treatment of HPV infection.