基于细胞外基质特性构建三维软骨纳米支架材料及表征

Fabrication and characterization of three-dimensional cartilage nano-scaffolds based on extracellular matrix

目的基于细胞外基质特性,制备三维软骨纳米支架材料。方法将水、三氟乙醇、六氟异丙醇混合作为溶剂,对Ⅱ型胶原(Col-Ⅱ)、玻璃酸(HA)、硫酸软骨素(CS)静电纺丝。测定支架材料的结构、形貌、热学性能、力学性能和疏水性。结果 Col-Ⅱ、HA、CS间存在相互作用,支架材料具有疏水性且保留Col-Ⅱ部分三螺旋结构。在研究范围内,支架纤维光滑细长,纤维直径分布集中。支架材料热稳定性良好,拉伸性能在质量比7∶1∶1时达到最优。结论制备的三维软骨纳米支架材料具有良好的热学、力学和结构特性,有望应用于软骨修复。

Objective To produce three-dimensional cartilage nanoscaffolds based on extracellular matrix. Methods Nanoscaffolds of collagen type Ⅱ（ Col- Ⅱ ） , hyaluronie acid （ HA ） and ehondroitin sulfate （CS） were prepared by mixing water, trifluoroethanol and hexafluoroisopropanol as a solvent. The structure, morphology, thermal property, mechanical performance and hydrophobicity of the scaffolds were characterized. Results There were interactions between Col-Ⅱ, HA and CS. The scaffolds were hydrophobic. The Col-Ⅱ triple-helix structure wasn＇t completely damaged. In the study, scaffold fibers were smooth, slender and dimensionally stable. The scaffolds had good thermal stability and optimal tensile properties could be obtained at the mass ratio of 7 ： 1 ： 1. Conclusion In this study, scaffolds have good thermal, mechanical and structural properties and are expected to be used in cartilage repair.