不同贮藏时间对文冠果成熟胚大小子叶诱导愈伤组织的影响

Effect Storage Time on Callus Induction from Big and Small Cotyledon in Mature Embryos of Xanthoceras sorbifolia

以2011—2015年不同贮藏年份的文冠果（Xanthoceras sorbifolia）种子为试材,研究了贮藏时间对成熟胚大小子叶诱导愈伤组织的影响,结果表明：2011年、2012年、2013年、2014年种胚小子叶诱导形成愈伤组织的诱导率均大于大子叶的;2015年种胚小子叶诱导形成愈伤组织的诱导率小于大子叶的;随着贮藏时间的延长,文冠果成熟胚愈伤组织的诱导率呈现出先下降后上升的规律,其中2012年贮藏（5年）的文冠果成熟胚大子叶诱导形成愈伤组织的诱导率为96.30%（MS培养基＋6-BA1.0mg/L＋2,4-Dl.0mg/L＋NAA0.5mg/L＋蔗糖30g/L＋琼脂6g/L）。

The materials in this study are from seeds of Xanthoceras sorbifolia with different storage time,and studied the effect on callus induction from big and small cotyledon in mature embryo,the results are as those as that follow：With the seeds stored in 2011,2012,2013 and 2014,the induction rate of calli induced by small cotyledon was greater than that induced by big cotyledon,With the extension of storage time,as a regular,the induction rates of mature embryo calli decrease firstly and then rise,and the degree of rise is lower than the degree of decline,Since 2012,It has been selected for five years,the induction rate of the mature embryo leaves was the highest in the explants,up to 96.30%（The optimal medium is MS＋6-BA1.0 mg/L＋2,4-Dl.0 mg/L＋NAA0.5 mg/L＋sucrose30 mg/L＋agar6 g/L）.