人胎肝中低分子天然抑瘤物对小鼠肉瘤S-180作用的研究

EFFECT OF LOW MOLECULAR WEIGHT NATURAL TUMOR SUPPRESSOR OF HUMAN FETAL LIVER ORIGIN ON THE GROWTH OF MOUSE SARCOMA CELLS IN VITRO AND IN VIVO

从人胎儿肝脏中分离出一种分子量较小的抑瘤物质(FLS-MeOH),观察了它对小鼠肉瘤S-180细胞生长的抑制效应。在体外琼脂培养中,当FLS-MeOH浓度达到300μg/ml时,可完全抑制S-180细胞的集落形成;给荷瘤小鼠每日注射FLS-MeOH8mg/g体重,共20d,可明显延长小鼠的存活时间,部分小鼠可以无病存活。这些结果说明FLSMeOH在体内外均有明显的抗肿瘤活性,而且具有分子量小、无组织和种系特异性,以及对肿瘤细胞不可逆的毒性作用等特性。

A low molecular weight natural tumor suppressor (FLS-MeOH) was isolated from human fetal liver and its suppressive effect on the growth of S-180 cells was studied both in vitro and in vivo. Under the condition of in vitro agar plate culture, spontaneous colony formation of S-180 cells was completely suppressed when the concentration of suppressor in culture medium reached 300μg/ml. Survival of the mice bearing S-180 cells and treated with intraperitoneal injections ot suppressor (8mg/d for 20 days) was obviously prolonged. 4 out of 10 treated mice were disease-free survivals for more than 100 days. All experimental facts indicate that the low molecular weight natural tumor suppressor of human fetal liver origin possesses the essential nature of anti-tumor activity but less harmful effect on the growth of normal hemopoietic cells.