延年益寿仙苓汤抗衰老作用的实验研究

Experimental Study on Anti-aging Effect of Yannian Yishou Xianling Decoction

目的研究延年益寿仙苓汤对小鼠抗衰老作用的机制,为其进一步开发和应用提供实验依据。方法将40只昆明种(KM)小鼠随机分为4组,即空白对照组(生理盐水组)、模型组、延年益寿仙苓汤组(治疗组)、维生素E组(Vit E组),进行饲养,每天早晚灌胃,每次0.2m L,持续灌胃50天。在第50天灌胃后用10%水合氯醛溶液麻醉小鼠,立即取小鼠血清、肾脏、大脑分别对其SOD活力、GSH-PX活力、MDA含量进行测定。结果与模型组比较,治疗组的SOD活力、GSH-PX活力均有提高、MDA含量均有下降。结论延年益寿仙苓汤有一定的抗衰老作用,且其机制与抗氧化作用有关。

Objective： To research into the anti-aging effects of Yannian Yishou Xiannling decoction on mice and provide experimental basis for its further development and application. Method： 40 KM mice were randomly divided into 4 groups： blank control group（ normal saline group）,model group,Yannian Yishou Xianling Decoction group（ treatment group）,vitamin E group（ Vit E group） and were fed. 50 days of continuous gavage were applied every morning and evening with 0. 2 ml each time. After the 50 th day of gavage,the mice were anesthetized with 10% chloral hydrate solution.The serum SOD,the activity of GSH-PX and the content of MDA were measured in serum,kidney and brain of the mice. Conclusion： The Yannian Yishou Xianling decoction has a certain anti-aging effect,and its mechanism is related to the antioxidant effect.