Lmcp对子宫内膜癌细胞Survivin蛋白活性的影响

The influence of Lmcp to the active of protein Survivin in endometrial cancer cells

目的:检测低分子柑橘果胶(low modified citrus pectin,Lmcp)对子宫内膜癌细胞株Ishikawa中凋亡调控因子Survivin蛋白表达的影响。方法:应用Western blot方法检测经不同浓度Lmcp(0、100、300、500nmol/L)作用不同时间(0、6、12、36 h)后,各组Ishikawa细胞中Survivin蛋白的表达情况。结果:提高Lmcp作用浓度及延长Lmcp作用时间,均可使Ishikawa细胞中Survivin蛋白的表达减弱。结论:Lmcp可下调子宫内膜癌细胞Ishikawa中存活蛋白Survivin的表达,且此效果有剂量(0~500 nmol/L)和时间(0~36 h)依赖性。

Objective：To detect the influence of Lmcp to expression of protein Survivin in endometrial cancer cells Ishikawa.Methods：To detect the expression of protein Survivin in endometrial cancer cells Ishikawa after being affected by Lmcp with different density range（0,100,300,500 nmol/L） and different time range（0,6,12,36 h） using the way of Western blot.Results：The expression of protein Survivin in endometrial cancer cells Ishikawa can be weakened by increasing affect density and affect time.Conclusion：Lmcp can weak the expression of protein Survivin in endometrial cancer cells Ishikawa with a dose（0~500 nmol/L） and time（0~36 h） dependent manner.