KCNQ1及SLC30A8基因多态性与2型糖尿病患者相关性研究

Correlation study between gene polymorphism of KCNQ1 and SLC30A8 and type 2 diabetes mellitus

目的分析KCNQ1及SLC30A8基因多态性与2型糖尿病(T2DM)患者的相关性。方法选取2015年9月~2017年9月间在本院确诊并接受治疗的T2DM患者100例作为观察组,选取同期在本院进行体检健康者100例作为对照组,收集两组对象早晨空腹静脉血5 m L,全自动生化分析仪检测糖化血红蛋白、空腹血糖水平、胰岛素和血脂的水平,基因型检测使用单碱基延伸法,分析不同基因位点和T2DM相关性状况。结果 SLC30A8基因对照组TT、CT及CC基因型的频率依次为16%、52%及32%,观察组依次为13%、49%、38%,差异无统计学意义(χ~2=1.290,P>0.05);对照组T、C等位的基因频率依次为61%、39%,观察组依次为73%、27%,差异有统计学意义(95%CI=0.795~1.684,OR=1.205,χ~2=11.553,P<0.05)。观察组TT基因型血清FINS及HOMA-B高于CT基因型和CC基因型,观察组TT基因型血清TC、TG和FPG低于CT基因型和CC基因型,差异均有统计学意义(P<0.05);KCNQ1基因对照组TT、CT及CC基因型的频率依次为27%、41%及32%,观察组依次为24%、45%、31%,差异无统计学意义(χ~2=2.006,P>0.05);对照组T、C等位的基因频率依次为53%、47%,观察组依次为64%、36%,差异有统计学意义(95%CI=0.863-2.104,OR=1.171,χ~2=12.995,P<0.05),CC基因型血清TC、TG、FPG及Hb A1c低于CT、TT基因型,血清FINS高于CT、TT基因型,差异均有统计学意义(P<0.05)。结论 SLC30A8基因多态性rs13266634位点和KCNQ1基因多态性rs2237892位点和T2DM发病及血管并发症有一定相关性。

Objective To analyze the correlation between KCNQ1 and SLC30 A8 gene polymorphisms and type 2 diabetes mellitus（T2 DM）. Methods 100 patients receiving treatment and diagnosed as T2 DM in our hospital from September2015 to September 2017 were selected as the observation group, and 100 healthy subjects undergoing physical examination in our hospital were selected as the control group during the same period. 5 m L of fasting venous blood were collected from participants of two groups, and detection of glycosylated hemoglobin, fasting blood glucose, insulin and lipid levels were analyzed by automatic biochemical analyzer. Genotype were detected by single base extension method. The correlation between different gene loci and T2 DM was analyzed. Results The gene frequencies of TT, CT and CC genotypes of SLC30 A8 were 16%, 52% and 32% in the control group, and were 13%, 49% and 38% in the observation group, respectively, with no significant difference（χ^2=1.290, P〉0.05）. The frequencies of T and C alleles were 61% and39% in the control group and were 73% and 27% in the observation group, where the difference was significant（95%CI=0.795-1.684, OR=1.205, χ^2=11.553, P〈0.05）. The serum FINS and HOMA-B levels of TT genotype in the observation group were higher than those in CT and CC genotype, while the levels of serum TC, TG and FPG in TT genotype in the observation group were lower than those in CT and CC genotype, where the differences were both significant（P〈0.05）. The gene frequencies of TT, CT and CC genotypes of KCNQ1 were 27%, 41% and 32% in the control group, and were 24%, 45% and 31% in the observation group, with no significant difference（χ^2=2.006, P〉0.05）. The frequencies of T and C alleles were 53% and 47% in the control group and were 64% and 36% in the observation group, where the difference was significant（95%CI=0.863-2.104,OR=1.171,χ^2=12.995,P〈0.05）. The serum TC, TG, FPG and Hb A1 c levels of CC genotype were lower than those in CT and TT genotype, while the levels of serum FINS in CC genotype were higher than CT and TT genotype, where the differences were both significant（P〈0.05）. Conclusion rs13266634 locus of SLC30 A8 polymorphism and rs2237892 locus of KCNQ1 gene polymorphism are correlated with T2 DM incidence and its vascular complications.