实时荧光定量PCR法检测口腔鳞状细胞癌组织和癌旁组织中幽门螺杆菌核酸

Detection of Helicobacter pylori nucleic acid in the cancerous and paracancerous tissues of oral squamous cell carcinoma by real-time quantitative PCR

目的了解口腔鳞状细胞癌(OSCC)组织和癌旁组织中幽门螺杆菌(Hp)的感染情况。探讨Hp与OSCC发生、发展的相关性。方法采用实时荧光定量聚合酶链反应(PCR)检测37例OSCC患者的癌组织和癌旁组织和32例正常口腔黏膜组织中的Hp核酸,并对实时荧光定量PCR阳性产物进行DNA测序分析。结果 37例OSCC患者的癌组织和癌旁组织中Hp核酸阳性9例(24.32%),正常口腔黏膜组织均为阴性,两者差异有统计学意义(χ~2=6.935,P=0.008)。在9例Hp核酸阳性的OSCC患者样本中,有8例癌旁组织检出Hp核酸,2例癌组织检出Hp核酸,差异有统计学意义(P=0.015)。结论部分OSCC组织和癌旁组织存在Hp感染,是否与OSCC的发生、发展存在相关性需进一步研究。

Objective To investigate the infection of Helicobacter pylori(Hp)in the cancerous and paracancerous tissue of oral squamous cell carcinoma. To explore the correlation between the Hp and the oral squamous cell carcinoma. Methods Real-time quantitative PCR(q PCR) detecting systemwas used to identify the Hp nucleic acid in carcinoma and paracancerous tissues of 37 patients with oral squamous cell carcinoma and normal mucosa of 32 patients with injuries of the jaws,and the positive product of q PCR was analyzed by DNA sequencing. Results QPCR showed that the positive rate of 24.32% of Hp nucleic acid in oral carcinoma and paracancerous tissues was significantly higher than that(0%)of normal control samples of the patients with injuries of the jaws(χ~2= 6.935,P = 0.008). Among the 9 Hp nucleic acid positive oral carcinoma cases,8 paracancerous samples were positive with Hp nucleic acid and 2 cancerous tissues were positive. This result also indicated a statistical significance(P = 0.015).Conclusions Hp infection exists in some cancerous and paracancerous tissue of oral squamous cell carcinoma,and there was an association between Hp infection and the occurrence and development of oral squamous cell carcinoma.