甲硫哒嗪对髓母细胞瘤DAOY细胞抑制作用研究

Study on thioridazine inhibiting the medulloblastoma cell line DAOY

目的探讨神经安定类药物甲硫哒嗪(Thioridazine,THIO)对于髓母细胞瘤的抑制作用机制。方法利用人髓母细胞系DAOY和正常细胞系研究THIO最佳的给药浓度。利用CCK8、Transwell和定量PCR的方法研究THIO对DAOY细胞移动能力、生存能力及肿瘤干细胞特性基因表达的影响。结果 10μmol/L THIO可抑制DAOY细胞增殖及其在低糖条件下的生存能力,降低DAOY细胞移动,减少肿瘤干细胞特性基因的表达。结论 THIO对髓母细胞DAOY有抑制作用,为临床治疗髓母细胞瘤提供了理论依据。

Objective To study the mechanism of inhibition effect of thioridazine （THIO） on medulloblas- toma. Methods DAOY cell line and normal cell lines were used to study the optimal concentration of THIO. The effect of THIO on the migration and survival ability of DAOY and the cancer stem cell genes expression was investiga- ted by using CCK8, transwell and qPCR. Results 10 μmol/L THIO could inhibite the proliferation and the survival a- bility under starvation condition of DAOY,and reduce the cell migration and the expression of cancer stem cell genes. Conclusion THIO has antitumor effect on medulloblastoma cell line DAOY, which can provide further evidence to explore the effect of THIO in clinical treatment.