白蛾周氏啮小蜂普通气味受体OR1的进化分析

Phylogenetic analysis on the olfactory receptor 1 gene of Chouioia cunea Yang

为了探究气味受体OR在白蛾周氏啮小蜂的嗅觉识别过程中的作用机制,利用Illumina Hiseq^(TM) 2000平台对其触角进行转录组测序,从转录组序列中得到了一个普通气味受体OR1的基因全序列,对其进行多角度的进化分析.结果显示,周氏啮小蜂OR1基因全长为1 257 bp,编码418个氨基酸.将周氏啮小蜂的OR1氨基酸序列与18种膜翅目昆虫进行比对的结果表明,周氏啮小蜂的OR1氨基酸序列与短管赤眼蜂和丽蝇蛹集金小蜂的一致性和相似性均较高,根据氨基酸序列构建的系统发育树结果也表明周氏啮小蜂与短管赤眼蜂和丽蝇蛹集金小蜂的亲缘关系接近.与寄主单一的对叶榕传粉榕小蜂相比,周氏啮小蜂OR1的同义突变率较低,密码子偏好性也相对较低,主要接受负选择压力.

To explore the mechanism of the olfactory receptor（OR） function in the process of olfactory recognition of Chouioia cunea Yang,the transcription sequencing was conducted on the tentacle of C. cunea by the Illumina Hi Seq TM2000. A common olfactory receptor OR1 gene was obtained,and its evolution was analyzed. The results showed that the length of CcOR1 was 1 257 bp,which encoded 418 amino acids. The amino acid sequence of OR1 of C. cunea was compared with the homologous sequences of 18 species of Hymenoptera,and the result showed that the consistency and similarity between the amino acid sequence of OR1 of C. cunea with the homologous sequences of Trichogramma pretiosum and Nasonia vitripennis were higher. The phylogenetic tree based on the amino acid sequences showed that the relationships between C. cunea with Trichogramma pretiosum and Nasonia vitripennis were close. Being compared with the host single Ceratosolen Solmsi Marchali,the synonymous mutation rate of OR1 of C. cunea was rather lower. The codons of CcOR1 had lower usage bias,and the gene was mainly negative selected.