甜菜BvM14-CMO、BvM14-BADH基因的克隆、盐胁迫表达及生物信息学分析

Cloning,salt stress expression and bioinformatics analysis of BvM14-CMO and BvM14-BADH genes

以耐盐甜菜(Beta vulgaris)M14品系为试验材料,利用RT-PCR克隆获得甜菜M14品系甜菜碱合成相关基因胆碱单加氧酶基因BvM14-CMO和甜菜碱醛脱氢酶基因BvM14-BADH的cDNA全长。荧光定量PCR测试结果表明,200和400 mmol·L^(-1)NaCl盐胁迫下BvM14-CMO及BvM14-BADH在甜菜M14品系的叶和根中均显著上调表达。通过蛋白质互作数据库预测,得到了拟南芥CMO和BADH蛋白的互作蛋白。采用甜菜M14品系盐胁迫转录组数据库,对甜菜M14品系中CMO和BADH互作蛋白的同源基因进行盐胁迫表达聚类分析,发现甜菜M14品系中大部分预测获得的CMO和BADH互作蛋白基因与BvM14-CMO和BvM14-BADH基因在盐胁迫下的表达变化趋势一致,这些研究结果为进一步深入研究BvM14-CMO和BvM14-BADH基因的功能奠定了理论基础。

Sugar beet（ Beta vulgaris） M14 was selected as an experimental material,and the full length cDNA of BvM14-choline monooxygenase and BvM14-betaine aldehyde dehydrogenase was cloned. The expressions of BvM14-CMO and BvM14-BADH were analyzed with RT-PCR. The results showed that expressions of BvM14-CMO and BvM14-BADH increased with both leaves and roots under 200 and 400 mmol·L-1 NaCl. Furthermore,the interaction protein of CMO and BADH protein in Arabidopsis thaliana was predicted by the protein interaction database. At the same time,the salt stress transcriptome database of sugar beet M14 was used to identify the homologous genes of CMO and BADH interacting proteins in M14. The expression clustering analysis exhibited that the expressions of most predicted interacting pro-tein genes were consistent with BvM14-CMO and BvM14-BADH in M14 under the salt stress. This study provided the theoretical basis for further study of 14 BvM14-CMO and BvM14-BADH function.