纳米钴对小鼠胚胎成纤维细胞的毒性

Cytotoxicity of cobalt nanoparticles to Balb/c3T3 mice fibroblasts

目的探讨纳米钴对小鼠胚胎成纤维细胞(Balb/c3T3)的毒性作用。方法将处于对数生长期的Balb/c3T3细胞暴露于分别暴露于30、50、100 nm的0(对照)、1、5、10、50、100μg/ml纳米钴培养液培养24 h。测定细胞活性、培养液上清中LDH的活力及细胞内ROS的含量。结果与对照组比较,10~100μg/ml的30 nm纳米钴染毒组和50、100μg/ml的50、100 nm纳米钴染毒组Balb/3T3细胞的存活率均较低,差异有统计学意义(P<0.05);且随着纳米钴染毒浓度的升高,不同粒径纳米钴染毒Balb/3T3细胞的存活率均呈下降趋势。与对照组比较,10~100μg/ml的30、50、100 nm纳米钴染毒组Balb/3T3细胞培养液中的LDH活力及细胞内ROS的含量均较高,差异均有统计学意义(P<0.05);且随着纳米钴染毒浓度的升高,不同粒径纳米钴染毒Balb/3T3细胞培养液中的LDH活力及细胞内ROS的含量均呈上升趋势。结论纳米钴对小鼠成纤维细胞的毒性作用与暴露剂量及颗粒直径有关,暴露剂量越大、颗粒直径越小,毒性越大。

Objective To investigate the toxic effects of cobalt nanoparticles（nano-cobah） on mouse embryonic fibroblasts（Balb/ c3T3）. Methods Balb/c3T3 cells were cultured in vitro and treated with nano-cobalt with different sizes （30 nm,50 nm and 100 nm） at the doses of 0μg/ml, 1μg/ml,5μg/ml, 10μg/ml,50μg/ml and 100μg/ml respectively for 24 h. The morphological changes of cells were observed and the cell viability and cell membrane integrity were detected by CCK-8 and LDH detection kit. The mechanism of cytotoxicity was analyzed by ROS level. Results Compared with the control group, the viabilities of Balb/3T3 cells in the 30 nm groups at 10-100μg/ml nano-cobalt, and 50 nm and 100 nm groups at 50 and 100μg/ml were significantly lower （P〈0.05） with a dose-dependent manner. Compared with the control group,the LDH activities and intracellular ROS contents were both significantly higher in the 30 nm, 50 nm and 100 nm groups at 10-100 μg/ml nano-cobah （P〈0.05）, with a close dependent manner. Conclusion The cytotoxicity of nano-cobalt to mouse fibroblasts is dose-dependent and size- dependent. The higher the exposure close is and the smaller the diameter of the particle is, the higher the toxicity will he.