饮酒对男性精子印记基因Peg3 DNA甲基化的影响

Effects of alcohol consumption on DNA methylation of imprinting gene Peg3 in human sperm

目的探讨饮酒对男性精子印记基因Peg3 DNA甲基化水平的影响。方法分别于2015年3—4月和2016年3—4月,在山西省妇幼保健院和山西医学科学院山西大医院不孕不育男科门诊中进行婚前检查或孕前检查的就诊者中选取213名健康育龄期男性作为研究对象,分为饮酒组(n=83)和非饮酒组(n=130)。通过结构式问卷,收集研究对象的行为习惯及生活方式等人口学信息资料。使用焦磷酸测序法检测精子印记基因Peg3 DNA甲基化水平。结果饮酒组的人群吸烟率和精子中Peg3 DNA的甲基化率均高于非饮酒组,差异有统计学意义(P<0.05);而2组人群年龄、水产品摄入情况、受教育水平和BMI间比较,差异均无统计学意义(P>0.05)。经多重线性逐步回归分析结果显示,排除混杂因素后,饮酒组男性精子Peg3 DNA甲基化水平较高(P<0.05)。结论饮酒可能会导致男性精子印记基因Peg3 DNA甲基化水平升高。

Objective To explore the effects of alcohol consumption on DNA methylation of imprinting gene Peg3 in human sperm. Methods A total of 213 reproductive-age men were recruited from the visits for premarital checkups and pre- pregnancy checkups at infertility clinic of Wemen Health Center of Shanxi and Shanxi Dayi Hospital,Shanxi Academy of Medical Sciences during March-April 2015 and March-April 2016,which were divided into alcohol group （n=83） and non- alcohol group （n =130）. Demographic information,including behavior and lifestyle,was collected by questionnaires. And methylation status on the DMRs of imprinting gene Peg3 was examined by bisulfite pyrosequencing in 213 participants. Results DNA methylation of imprinting gene Peg3 in human sperm and smoking consumption were significantly higher in alcohol group compared with those in non-alcohol group （P〈0.05）. However, there were no significant difference in age, intake of aquatic products, education and BMI between alcohol group and non-alcohol group （P〉0.05）. Multiple linear regression analysis showed that after excluding selected confounding factors,alcohol consumption was positively correlated to DNA methylation of imprinting gene Peg3 in human sperm （P〈0.05）. Conclusion Alcohol consumption is associated with higher level of DNA methylation of imprinting gene Peg3 in human sperm.