基于高分辨质谱的180天受控生态密闭系统环境人体小分子代谢物变化规律研究

Research on Changes of Small Molecule Metabolites in Human under 180-day CELSS Environmental Conditions with High Resolution Mass Spectrometry

目的制定科学合理的营养保障措施,探讨受控生态密闭系统环境下营养代谢变化规律。方法招募4名受试者进入密闭舱开展180天受控生态环境实验,实验期间分别收集4名受试者的晨尿。运用高分辨质谱(UHPLC-Q/Exactive)检测尿液中的小分子代谢物,建立偏最小二乘判别分析模型筛选密闭环境下显著变化的代谢物,研究受控生态密闭系统环境对人体营养代谢的影响,并通过代谢通路分析识别密闭环境下的代谢变化。结果共有70个小分子代谢物发生了显著的变化,反映了密闭环境下对人体蛋白分解、能量代谢、脂质氧化、肠道菌群结构等的影响。结论长期受控生态密闭系统环境下人体小分子营养代谢产物发生了变化,需要有针对性的采取营养防护措施。

Objective To establish nutritional protection measures scientifically and rationally,by exploring the characteristics of nutritional metabolism in long-term confined environment. Methods Four volunteers were recruited to participate in the 180 d CELSS integration experiment. Morning urine were collected from the four volunteers during the experiment. UHPLC-Q/Exactive high resolution mass spectrometry was used to detect the small molecular metabolites in the urine. The partial least squares discriminant analysis model was used to select the metabolites with significant changes in the confined environment. The effects of the enclosed environment on the nutrient metabolism were studied. Identification of metabolic variation in the confined environment was analyzed by metabolic pathway analysis. Results A total of 70 small molecule metabolites changed significantly,reflecting the impact of confined environment on human protein digestion,energy metabolism,lipid oxidation,intestinal microflora structure and so on. Conclusion The small molecule nutrient metabolites changed during the resident in long-term controlled environment,and it is necessary to adopt nutrition protection measures.