微生物来源的缩肽类化合物enopeptin A的研究(Ⅰ)：菌种选育及发酵工艺研究

Study on depsipeptide compound enopeptin A derived from microorganisms:its breeding and fermentation technology(Ⅰ)

Enopeptin A是一种缩酚酸肽类化合物,具有抗菌、抗病毒等生物活性。以本实验室保藏的链霉菌SIIA-H7264为出发菌,通过紫外(UV)照射、甲基磺酸乙酯(EMS)诱变、亚硝基胍(NTG)诱变以及常压室温等离子体(ARTP)诱变,获得1株高产突变株A-21,其发酵产物Enopeptin A的产量较出发菌株提高了3倍。对发酵条件、发酵培养基组分进行单因素及响应面优化试验,最终Enopeptin A的产量达到1750μg/m L,较初始工艺提高了2.98倍。

Enopeptin A is a depsipeptide with antibacterial, antiviral and a variety of biological activities. The original strain SIIA-H7264 was mutated by UV, NTG, EMS and atmospheric temperature plasma. A high yield and good genetic stability strain A-21 was obtained with a titer of three folds higher than the original strain. The fermentation conditions and fermentation medium were studied by the Plackett-Burmen experiment, the climbing tests and the Box-Behnken method. Under the optimum medium and conditions, the yield of enopeptin A was 1750pg/mL and 2.98 folds higher than that with the initial conditions.