牛支原体中国株的多位点序列分型

MLST of Mycoplasma bovis isolates from China

为了解中国牛支原体的流行趋势，本试验应用已经建立的牛支原体分型方法，将中国的15株牛支原体进行MI。ST分型，同时结合数据库中已有的11株中国株的ST型，分析牛支原体中国株的种群结构及进化关系。分型结果显示，15株分离株均获得一样的等位基因号及ST型；共计26株中国株分属于3个ST型，即R—ST10（16／26），R—ST33（9／26），RST34（1／26），而且彼此之间只有1个等位基因的差异，10型和33型之间的差异是rpoD基因，33型和34型之间的差异是danA基因；说明目前牛支原体中国株的种群结构相对单一，流行菌株相对稳定。

In order to understand the popular trend of Chinese Mycoplasma bovis , the experiment in this study employed the established multilocus sequence typing（MLST） methods in the Ro sales＇ study to type 15 Mycoplasma boris isolates from China. The population structure and evo- lutionary relationship of the Chinese M. boris isolates were analyzed by combining the existing STs of 11 Chinese isolates in the database. Fifteen samples got the same allele number and se- quence type（ST）. A total of 26 Chinese isolates fall into three STs, ST10 （16/26）,ST33 （9/26）, and ST34（1/26）. In addition,there is only one allelics difference between each ST. It is rpoD gene between ST10 and ST33 and danA gene between ST33 and ST34. These results indicate that the population structure of Chinese M. boris isolates is simple and the epidemic strains are stable to some extent.