细针型共聚焦激光显微内镜在体成像的动物实验研究

Imaging of needle-based confocal laser endomicroscopy in rabbit models

目的探索细针型共聚焦激光显微内镜（nCLE）对动物腹腔组织及脏器成像的可行性。方法选取5只实验家兔的大网膜、肝脏、胰腺、腰大肌（骨骼肌）为研究对象，nCLE经由19G穿刺针进入其内部分别进行成像，观察各腹腔组织及脏器的成像特征，并留取清晰的nCLE图像。最终将nCLE图像与病理组织学结果相比对。结果5只实验家兔腹腔组织及脏器的nCLE成像均成功完成，并清晰显示大网膜、肝脏、胰腺、腰大肌（骨骼肌）的细胞、腺体、微血管等显微结构，其nCLE图像具有特征性。各腹腔组织及脏器nCLE图像与病理组织学具有良好的一致性。结论利用nCLE对腹腔组织及脏器成像具有可行性。

Objective To evaluate the feasibility of needle-based confocal laser endomicroscopy （nCLE） for imaging of intra-abdominal tissues and organs in rabbit models in vivo. Methods The nCLE miniprobe was inserted through the 19-gauge needle into various intra-abdominal tissues and organs[ omentum majus, liver, pancreas and psoas major （skeletal muscle）]. The nCLE images were acquired and real-time sequences of respective locations were recorded. Finally, nCLE image characteristics were compared with histopathologic findings. Results nCLE was successfully performed in intra-abdominal tissues and organs of five rabbit models. The microscopic structures of cells, glands and microvessels in the omentum majus, liver, pancreas and psoas major （ skeletal muscle） were visualized clearly, respectively. Characteristics of various intra-abdominal tissues and organs were displayed on nCLE images, which were correlated well with histological findings. Conclusion hnaging of intra-abdominal tissues and organs with nCLE in vivo is feasible in future clinical practice.