摘要
研究百里香中抑菌成分的超高压提取工艺及抑菌作用,考察压力、酒精浓度、料液比对百里香提取物抑菌作用大小的影响,采用响应面法优化百里香抑菌成分超高压提取的最佳条件;采用倍半稀释法测定百里香提取物对金黄色葡萄球菌、大肠杆菌及单增李斯特菌的最小抑菌浓度(MIC);检测抑菌物质对细菌的生长曲线、核酸泄漏的影响;对百里香提取物成分进行分析。实验结果表明:在压力380MPa、乙醇浓度83%、料液比1∶42(g/mL)的条件下,相对电导率为15.69%±0.21%;百里香提取物处理细菌后与对照组相比,菌生长缓慢,迟滞期延长,细胞膜通透性增加,核酸泄露量明显增加,说明百里香提取物对金黄色葡萄球菌、大肠杆菌及单增李斯特菌生长具有一定抑制作用;每1g百里香超高压提取物中含黄酮0.189mg、多酚0.162mg、多糖0.085mg、百里香酚0.134mg。
Ultra high pressure extraction and bacteriostasis of the antibacterial components in thyme are studied,the effects of pressure,alcohol concentration and ratio of material to liquid on the antibacterial activities of the extracts are studied by response surface methodology.The antibacterial activities(MIC)of Staphylococcus aureus, Escherichia coli and Listeria monocytogenes are determined by semi-dilution method.The influences of antibacterial substances on the growth curve and leakage rate of nucleic acid of bacteria are investigated,and the constituents of the extracts of thyme are analyzed.The results suggest that the optimal extraction conditions are determined as follows:extraction pressure is 380 MPa,ethanol concentration is 83%,solid to liquid ratio is 1∶42(g/mL).Under these optimized conditions,the relative conductivity is 15.69% ±0.21%,compared with the control group,the growth of bacteria is slow,the hysteresis period is prolonged,the membrane permeability is increased,and the leakage rate of nucleic acid is increased obviously,which indicate that the extracts from thyme have inhibitory effects on the growth of Staphylococcus aureus,Escherichia coli and Listeria monocytogenes.The ultra high pressure extracts of thyme contain flavonoids of 0.189 mg,polyphenols of 0.162 mg,polysaccharides of 0.085 mg,thymol of 0.134 mg.
作者
王娣
柯春林
曹珂珂
李妍
WANG Di;KE Chun-lin;CAO Ke-ke;LI Yan(Department of Biology and Food Engineering, Bengbu University, Bengbu 233030, Chin)
出处
《中国调味品》
CAS
北大核心
2018年第4期93-100,共8页
China Condiment
基金
安徽省教育厅重点科研项目(KJ2015A204)
关键词
百里香
超高压提取
抑菌作用
电导率
thyme
ultra high pressure extraction
antibacterial activity
conductivity