桑葛降糖粉的生产工艺与体外活性研究

Study on the production process and in vitro activity of Sang Ge powder

桑葛降糖粉是当下正在研究的一种新型的降血糖保健食品,以多糖和异黄酮为主要有效成分。本文综合考虑药材中的降血糖活性成分和实际生产,选取桑叶和葛根的最佳提取工艺,并对产品进行体外活性验证。分别以桑叶多糖和葛根素的得率及干膏得率为指标,通过正交实验确定桑叶和葛根的最佳提取工艺。以总多糖对α-葡萄糖苷酶的抑制活性为指标考察桑葛降糖粉的体外活性。结果表明:桑叶的最佳提取工艺为50倍量水于80℃浸取3次,每次提取1.0 h;葛根的最佳提取工艺为12倍量70%乙醇,80℃水浴,提取2次,每次2 h,经过验证,桑叶多糖的得率为3.86%,葛根素的得率为26.30%,提取效果较好。对α-葡萄糖苷酶的半抑制浓度(IC_(50))分别是:阿卡波糖为0.0648 g/L,桑葛降糖粉中的总多糖为0.1862 g/L,桑叶多糖为0.3798 g/L,桑葛降糖粉呈现出对α-葡萄糖苷酶良好的活性抑制作用,且与桑叶多糖比较,其活性显著提高(p<0.01)。生产工艺的确定和体外活性结果为桑葛降糖粉的深入研究及生产奠定了基础。

Sang Ge powder is a new anti-diabetic health food which is developing. Main active ingredients of it are polysaccharides and isoflavones. To study the best extractions process of mulberry leaves and pueraria by analysising the hypoglycemic active components of the medicinal material and the actual production. Verified the in vitro activity of the products.The optimum extraction techniques of mulberry leaves and pueraria were determined by orthogonal test,the yield of polysaccharides and puerarin and the rate of dry paste were used as the index of evaluation. The anti-diabetic activity was verified by measuring the α-glucosidase inhibitory activity of total polysaccharides from Sang Ge powder.The results showed that the best extraction process of mulberry leaves was 50 times of water at 80 ℃ for 3 times,and the extraction time was 1.0 h.The best extraction process of pueraria was 12 times of 70% ethanol,80 ℃ water baths for 2 times,and each time was 2 h.The yield of mulberry leaves was 3.86% and of puerarin was 26.30% after verification.Showed the extraction effect was better.The halfmaximal inhibitory concentration（ IC_（50）） value of Acarbose was 0.0648 g/L,the total polysaccharide of Sang Ge powder was0.1862 g/L,the mulberry leaf polysaccharide was 0.3798 g/L. Sang Ge powder showed a good inhibitory effect onα-glucosidase,and the activity was significantly higher than that of mulberry leaf polysaccharide（ p 0.01）. The production process and in vitro activity study laid the foundation for the future development of Sang Ge powder.