NF-κB在小鼠胚胎着床前植入期子宫内膜的表达研究

The Study on NF-κB Expression in the Endometreum of Mice Embryo Implantation at the Stage of Pre-Implantation

核转录因子NF-κB(nuclear factor of kappa B)是参与炎症反应的重要转录因子,而胚胎着床这一生理过程类似于炎症反应。该研究通过Real-time PCR、Western blot以及免疫组织化学等方法检测了妊娠小鼠孕第1天(d1)至第5天(d5)子宫内膜NF-κB的表达情况,并通过ELISA方法检测了妊娠小鼠(孕d1~d5)血清中白细胞介素-6(interleukin-6,IL-6)与肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的含量。研究发现,NF-κB于孕d1开始表达,且随着妊娠天数的增加表达呈现逐渐上升的趋势,其m RNA水平与蛋白质水平相一致。NF-κB在孕d5于子宫内膜表达着床点高于着床旁,而在假孕小鼠子宫内膜中NF-κB的表达低于真孕组,炎症因子IL-6与TNF-α的含量也随妊娠天数的增加表达逐渐上升。该研究结果表明,NF-κB可能通过炎症反应的过程参与了胚胎着床这一生理过程。

NF-κB （nuclear factor of kappa B） is an important nuclear transcription involved in the inflammatory response, and embryo implantation is a physiological process is similar to the inflammation. This study examined the expression of NF-κB in endometrium of pregnancy mice （d1-d5） by Real-time PCR, Western blot and immunohistochemistry, and we detected the content of interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） in serum of pregnancy mice （d1-d5） by ELISA method. The study found that NF-κB was expressed on the first day of pregnancy and showed a gradual increased with the number of days of pregnancy. NF-κB was expressed in the endornetrium of pseudopregnant mice and which was lower than that in the endometrium of pregnant mice at 5 d, and the expression of inflammatory cytokines IL-6 and TNF-α also increased with the number of the pregnancy days. The results of this study indicated that NF-κB may participate in the physiological process of embryo implantation through the process of inflammatory reaction.