注射用益气复脉(冻干)对辐射所致小鼠免疫低下的改善作用

Effect of Yiqi Fumai Lyophilized Injection on immune suppression induced by irradiation in mice

目的研究注射用益气复脉(冻干,YQFM)对^(137)Cs辐照后小鼠免疫系统的影响。方法按体质量将昆明小鼠随机分为5组:对照组、模型组和YQFM低、中、高剂量(354.55、709.09和1 418.18 mg/kg)组,每组10只。除对照组外,其余各组小鼠放在圆形有孔的照射盒中采用^(137)Cs全身1次性照射剂量为3 Gy的射线,制备免疫低下小鼠模型,于照射24 h后,尾iv给药,每天1次,连续6 d,对照组和模型组尾iv同体积辅料溶液。检测小鼠胸腺指数;ip 5%的鸡红细胞悬液检测血清溶血素的含量;耳廓迟发型超敏反应模型采用2,4-二硝基氯苯制备,以小鼠耳肿胀度为观测指标;采用动物血液分析仪测定小鼠骨髓有核细胞(BMNC)和白细胞(WBC)数;用全自动生化仪测定血清中尿素氮(BUN)和丙氨酸转氨酶(ALT)的含量变化。结果与模型组比较,YQFM各剂量组均可显著增加免疫低下小鼠的胸腺指数、显著增强迟发型超敏反应和增加BMNC、WBC数(P<0.05、0.01);低、高剂量组显著增加血清溶血素水平(P<0.01);低剂量组显著减少血清中ALT含量(P<0.05)。结论 YQFM可显著改善^(137)Cs所致的小鼠免疫低下,有可能成为一种新型的抗肿瘤辅助用药。

Objective To study the effect of Yiqi Fumai Lyophilized Injection（YQFM） on immune system of mice after exposure to 137Cs irradiation. Methods KM mice were divided into five groups： control group, model group, YQFM low, medium, high dose（354.55, 709.09 and 1 418.18 mg/kg） groups, with 10 mice in each group. Except for control group, mice in remaining groups were placed in a circular perforated irradiation box using 137Cs systemic irradiation dose of 3 Gy radiation, preparing immune compromised mouse model. After 24 h exposure, drugs were administrated in tail vein continued for six days, once a day. Mice in control and model group were injected with the same volume of intravenous infusion solution. Thymus index of mice was detected.Detection of serum hemolysin level was taken by ip injection of 5% chicken erythrocyte suspension. Using 2, 4-dinitrochlorobenzene to establish the delayed type hypersensitivity model, and the changes of ear swelling degree were observed. Moreover, the number of bone marrow nucleated cells（BMNC） and leucocyte（WBC） in mice were determined by animal blood analyzer. Change of the level of serum Urea nitrogen（BUN） and alanine amino transferase（ALT） were measured by automatic biochemical analyzer. Results Compared with model group, low, medium and high doses of YQFM significantly increased the thymus index in immune compromised mice, and significantly enhanced delayed type hypersensitivity and increased the number of BMNC and WBC（P 〈0.05、0.01）; YQFM at the low and high doses increased the production of hemolysin; Low dose of YQFM significantly decreased the level of serum ALT（P 〈 0.05）. Conclusion YQFM can markedly enhance the immune function in mice with immunodeficiency induced by 137Cs, and is expected to become a novel antineoplastic assistant drug.