自身免疫性胰腺炎患者血清外泌体中CD69表达升高及意义

Elevated Expression of CD69 in Serum Exosomes from Patients with Autoimmune Pancreatitis and Its Significance

目的探究自身免疫性胰腺炎(AIP)患者血清外泌体中CD69的表达情况,初步探讨其对疾病病理过程的影响。方法收集2012年10月~2016年12月来长海医院就诊的35例AIP患者血清和同期就诊的35例健康个体血清,分别作为实验组和对照组。以ELISA法检测血清中的细胞因子浓度,以磁珠结合抗体的方法在流式细胞仪中检测血清中外泌体表面分子的表达情况,两组之间计量资料的比较采取两独立样本t检验,两变量之间的相关性以Pearson系数表示。结果流式细胞仪分析显示,实验组血清中外泌体CD69表达水平高于对照组(85.76±19.45vs 17.01±5.89)%,差异有统计学意义(t=20.01,P<0.0001);实验组中CD69+的外泌体所占百分比与血清中IL-4,IFN-γ,TGF-β表达量呈正比(r=0.456,0.678,0.548,均P<0.05),CD69-的外泌体所占百分比与血清中IL-4,IFN-γ,IL-10,TGF-β呈反比(r=-0.589,-0.399,-0.784,-0.657,均P<0.05)。结论 CD69在外泌体的表达可能通过影响CD4+T细胞功能参与了AIP病程,是该病潜在的监测指标和治疗靶点。

Objective To study the expression of CD69 in exosomes existed in serum from patients with autoimmune pancreatitis（AIP）and primarily discuss its affection on the pathology of disease progression.Methods Serum samples from 35 cases diagnosed as AIP and 35 healthy individuals examined at the same time were collected during October 2012 to December 2016 in Changhai Hospital,they were defined as experimental and control groups,separately.The cytokine levels in serum were measured by ELISA.The expression of surfaced molecules in exosomes existed in serum was tested by magnetic beadconjunct-antibody method in flow cytometry.The comparison between the two groups＇ measurement data was measured by two independent samples＇ t test,correlation between two variables was showed by Pearson coefficient.Results The flow cytometry analysis showed that the expression level of CD69 in serum exosomes in experiment and control groups were（85.76±19.45 vs 17.01±5.89）%,with statistical difference（t=20.01,P〈0.0001）.CD69＋ exosomes in experiment group were positively related with serum IL-4,IFN-γand TGF-β（r=0.456,0.678,0.548,all P〈0.05）,while CD69-exosomes were negatively related with serum IL-4,IFN-γ,IL-10 and TGF-β（r=-0.589,-0.399,-0.784,-0.657,all P〈0.05）.Conclusion The expression of CD69 in exosomes might participate in AIP progression through influencing the function of CD4＋T cell,and it was the potential examined biomarker and therapeutic target.