COX-2和survivin蛋白在非小细胞肺癌中的表达情况及临床意义

The expression and clinical significance of COX-2 and survivin protein in nonsmall cell lung cancer

目的探讨环氧化酶-2(COX-2)和凋亡抑制蛋白(survivin)在非小细胞肺癌中的表达情况及对预后的影响。方法选取85例非小细胞肺癌患者,收集患者的肿瘤组织及癌旁组织,通过免疫组织化学染色法检测两种组织中COX-2和survivin蛋白的表达情况,分析其与患者临床特征的关系及预后情况。结果非小细胞肺癌组织中COX-2和survivin的平均光密度均高于癌旁组织,差异均有统计学意义(P﹤0.05);COX-2的阳性表达率为64.71%,survivin的阳性表达率为58.82%;COX-2和survivin的阳性表达率在不同年龄和性别的非小细胞肺癌患者中比较,差异均无统计学意义(P﹥0.05);肿瘤直径≥4 cm、低分化、Ⅲ~Ⅳ期、有淋巴结转移和有吸烟史患者的COX-2和survivin阳性表达率明显高于肿瘤直径﹤4 cm、中高分化、Ⅰ~Ⅱ期、无淋巴结转移和无吸烟史的患者(P﹤0.01)。COX-2和survivin阳性表达的非小细胞肺癌患者的中位生存期均明显短于阴性表达的患者(P﹤0.01)。结论 COX-2和survivin蛋白在非小细胞肺癌组织中高表达,可能参与了非小细胞肺癌的发生和发展,COX-2和survivin高表达患者的预后较差。

Objective To observe the expression and prognostic significance of cyclooxygenase-2（COX-2） and survivin protein in non-small cell lung cancer（NSCLC）. Method 85 patients with NSCLC were collected for the cancer tissues and adjacent tissues, immunohistochemical staining was used for detecting the expression of COX-2 and survivin protein in these specimens to analyze the relationship with patients＇ clinical characteristics and prognosis. Result The average optical density of COX-2 and survivin in NSCLC tissues were higher than those in adjacent tissues（P〈0.05）;the positive expression rate of COX-2 was 64.71%, and that of survivin was 58.82%; the positive expression rate of COX-2 and survivin were of no statistically significant difference in NSCLC patients with different age and gender（P〈0.05）; while tumor diameter ≥4 cm, poor differentiation, stage III-IV disease, with lymph node metastasis and smoking history were associated with higher positive expression of COX-2 and survivin as compared with those with tumor diameter 4 cm, moderately to highly differentiated, stage I-II disease, no lymph node metastasis and were non-smokers（P〈0.01）. The median survival time of NSCLC patients with positive COX-2 and survivin expression was significantly lower than those with negative expression（P〈0.01）. Conclusion The expression of COX-2 and survivin protein are significantly higher in patients with NSCLC, which may be involved with the occurrence and development of NSCLC, besides, high expression of COX-2 and survivin protein may predict poor prognosis.