摘要
The sensitive and selective detection of nucleic acids is important for basic research and many applied fields. Herein, a novel green DEAB-TO-1 was designed and synthesized. DEAB-TO-1 shows significant fluorescence enhancement(800-fold) when bound to ctDNA and a distinct selectivity for dsDNA over RNA(5.9 times). Moreover, the limit of detection is 0.57 ng/ml. It is live-cell-permeant DNA intecalator,indicating a promising candidate for nucleus-specific imaging and a good counter-stain compatibility with other dyes.
The sensitive and selective detection of nucleic acids is important for basic research and many applied fields. Herein, a novel green DEAB-TO-1 was designed and synthesized. DEAB-TO-1 shows significant fluorescence enhancement（800-fold） when bound to ctDNA and a distinct selectivity for dsDNA over RNA（5.9 times）. Moreover, the limit of detection is 0.57 ng/ml. It is live-cell-permeant DNA intecalator,indicating a promising candidate for nucleus-specific imaging and a good counter-stain compatibility with other dyes.
基金
supported by the National Natural Science Foundation of China(21421005,21576037,21422601,21406028,U1608222)
