MiR-296-5p在弥漫大B细胞淋巴瘤中的表达及其对肿瘤细胞生物学行为的影响

Expression of MiR-296-5p in Diffuse Large B-Cell Lymphoma and Its Influence on Biological Behavior of Tumor Cells

目的:检测弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)细胞中micro RNA-296-5P(mi RNA-296-5p)的表达水平,并研究干扰mi RNA-296-5p的表达对DLBCL细胞株DB的增殖、迁移及凋亡的影响。方法:应用人淋巴细胞分离液提取健康人外周血单个核细胞,实时荧光定量PCR检测单个核细胞与DLBCL-DB细胞mi RNA-296-5p的表达。利用mi RNA-296-5p inhibitor干扰DB细胞mi RNA-296-5p的表达,实时荧光定量PCR检测转染后mi RNA-296-5p的表达,并进一步用CCK-8法、Transwell法和Annexin V-FITC/PI双染法检测转染后细胞增殖、迁移和凋亡的变化。结果:mi RNA-296-5p在DLBCL细胞株DB中明显高表达,转染mi RNA-296-5p inhibitor后细胞的增殖和迁移能力明显受到抑制,而细胞凋亡没有明显改变。结论:mi RNA-296-5p的高表达可能与DLBCL的发生发展有关,可能成为DLBCL的预后评估指标之一。

Objective： To detect the expression of miRNA-296-5p in diffuse large B cell lymphoma （DLBCL） cells and study the proliferation, migration and apoptosis of DLBCL cells after interfering the expression of miRNA-296-5p. Methods： The expression of miRNA-296-5p in DLBCL cells line DB cells was detected by real-time quantitative reverse -transcription polymerase chain reaction （qRT-PCR）. The possible roles of miRNA-296-5p in the biological and behavioral properties of DLBCL - DB cells were explored by transfection of miRNA-296-5p inhibitor for miRNA-296-5p knockdown, and detected by using CCK-8 method, Transwell method and Annexin V-FITC/PI. Results： miRNA-296- 5p was highly expressed in DLBCL - DB cells, and the inhibition of miRNA-296-Sp could induce suppression of cell proliferation and migration, but the cell apoptosis was not changed significantly. Conclusion：The high expression of miRNA-296-5p may relate with the occurence and developmentof DLBCL, which may be a new therapeutic target for DLBCL.