脂肪血制备的去白细胞悬浮红细胞储存期代谢特性研究

Metabolic Characteristics of Leucocyte-deplated and Suspended RBC Produced by Using Lipid Whole Blood During Routine Storage

目的:探讨脂肪血制备的去白细胞悬浮红细胞在储存期的主要代谢途径转换,为脂肪血制备的悬浮红细胞储存提供参考。方法:选择脂肪血和正常全血各10 U,制备成去白细胞悬浮红细胞,在制备当天(d 0)、d 14、d 35分别取样,采用UPLC-MS/MS检测红细胞内的代谢物。采用Mann-Whitney U检验分析2组标本糖酵解途径和磷酸戊糖途径代谢物的差异,分析脂肪血制备的悬浮红细胞储存期的代谢途径变化。结果:d 0实验组红细胞内代谢物葡萄糖、腺嘌呤、丙酮酸、GSH、GSSG和烟碱酰胺水平均高于对照组(P<0.05)。d 14实验组红细胞中葡萄糖、丙酮酸和GSH水平低于对照组(P<0.05),腺嘌呤、烟碱酰胺和GSSG水平高于对照组。D 35实验组红细胞中葡萄糖水平低于对照组(P<0.05),腺嘌呤、烟碱酰胺水平高于对照组(P<0.05),两组之间的丙酮酸、GSSG和GSH水平差异无统计学意义(P>0.05)。结论:与正常红细胞相比,脂肪血红细胞储存期内能量代谢减弱,磷酸戊糖途径增强。

Objective： To investigate the differences of metabolic pathways of leucocyte-deplated RBCs prepared by using lipid whole blood and nomal blood during routine storage so as to provide some reference for clinical blood use. Methods： Twenty U whole blood from 20 donors, including 10 U lipid blood and 10 U normal whole blood, were selected for preparing leukodepleted red blood cells, red blood cells were taken from storage bags on day 0, 14 and 35, respectively. Metabolites in the red blood cells were analyzed, red blood cell metabolic extracts were detected by UPLC - MS/MS. The metabolite data of RBC from 2 groups were analyzed by SIMCA - P 13.0 software using OPLS - DA and by SPSS 19.0 using Mann-Whitney U test. Difference of metabolic pathways was described according to different metabolites. Results： The glucose, adenine, pyruvic acid, GSH, GSSG and niacinamide levels on day 0 in lipid RBCs were higher than those in the control group（ P 〈 0.05 ）. The glucose, pyruvic acid and GSH levels on day 14 in lipid RBCs were lower than those in the control group （ P 〈 0.05 ）, and the levels of adenine, GSSG and niacinamide were higher than that in the control group （ P 〈 0.05 ）. The glucose level on day 0 was lower than that in the control group （ P 〈 0.05 ）, and the levels of adenine and niacinamide were higher than those in the control group （ P 〈 0.05 ）. but the pyruvic acid, GSH and GSSG levels were not significantly different between 2 groups （ P 〉 0.05 ）. Conclusion ： Compared with the normal red blood cells, the energy metabolism pathway decreases in lipid red blood cells within the storage period and pentose phosphate pathway increases.