高效液相色谱法测定金刚藤口服液中薯蓣皂苷元的含量

Quantification of Diosgenin in Smilax oral liquid by HPLC

建立高效液相色谱法测定金刚藤口服液中薯蓣皂苷元的含量。色谱条件:色谱柱为Kromasil C18 150×4.6 mm 5μm;Kromasil C18200×4.6 mm 5μm;Symmetryshield C18 150×3.9 mm 5μm;流动相为乙腈-水(90∶10);流速1.0 mL/min;检测波长205 nm;柱温35℃;进样量10μL、20μL。线性范围为0.55~5.5 mg/mL(r=0.9999),加标平均回收率为96.31%,RSD为2.06%。本方法准确度高、精密度高、重复性好、简捷易操作,可以作为金刚藤口服液中薯蓣皂苷元含量的测定方法。

The objective of this study was to establish a HPLC method for analyzing the content of diosgenin in Smilax oral liquid. The chromatographic conditions were as follows： column Kromasil C18（150×4.6 mm 5μm）; Kromasil C18（200×4.6 mm 5μm）; Symmetryshield C18（150×3.9mm 5μm）; mobile phase acetonitrile-water （90 ： 10）; flow rate 1.0 mL/min; detection wavelength 205 nm; column temperature 35℃; injection volume 10μL, 20μL. The method was linear within the range of 0.55-5.5 mg/mL （r =0.9999）, average recovery is 96.31%, RSD of 2.06%. This method is accurate, precise, repeated and easy to operate. It can be used for analyzing content ofdiosgenin in Smilax oral liquid.