白细胞介素-6对子宫颈癌细胞株C-33A增殖影响的研究

Research on the effects of interleukin-6 on proliferation of cervical cancer cell C-33A

目的探讨白细胞介素-6(interleukin-6,IL-6)在子宫颈癌中的作用。方法将培养后的子宫颈癌细胞C-33A分为IL-6组和对照组,IL-6组使用IL-6(50 ng/m L)刺激,对照组不加入IL-6,利用MTT比色法检测细胞增殖,划痕实验检测细胞迁移,实时荧光定量聚合酶链反应技术检测上皮钙黏着蛋白(epithelial-cadherin,ECad)、神经钙黏着蛋白(neural-cadherin,N-Cad)、波形蛋白(vimentin)、转录因子Snail1(transcription factorssnail1,TFs-SNAIL1)在m RNA水平上的表达,蛋白质印迹法检测E-Cad、N-Cad、Vimentin、TFs-SNAIL1蛋白水平的表达。结果与对照组比较,IL-6组子宫颈癌细胞株C-33A增殖活性更高(12 h:0.388±0.025 vs.0.597±0.057;24 h:0.547±0.021 vs.0.798±0.036;48 h:0.745±0.056 vs.1.296±0.122;72 h:1.074±0.053 vs.1.805±0.113;P<0.05),迁移能力更强(12 h:1.057±0.029 vs.1.200±0.045;24 h:1.189±0.036 vs.1.428±0.181;48 h:1.273±0.059 vs.1.569±0.143;72 h:1.409±0.047 vs.1.623±0.170;P<0.05),E-Cad m RNA及蛋白的表达更低(1.012±0.098 vs.0.483±0.171,P<0.01;1.032±0.015 vs.0.395±0.119,P<0.01),N-Cad m RNA及蛋白表达更高(1.054±0.106 vs.1.465±0.230,P<0.01;1.040±0.043 vs.1.605±0.128,P<0.01),vimentin m RNA及蛋白表达更高(1.050±0.083 vs.1.340±0.099,P<0.05;1.043±0.062 vs.1.430±0.077,P<0.05),TFs-SNAIL1 m RNA及蛋白表达更高(1.058±0.176 vs.1.510±0.229,P<0.01;1.022±0.015 vs.1.470±0.139,P<0.01)。结论 IL-6可能促进子宫颈癌细胞株C-33A的增殖、迁移和上皮-间质转化。

Objective To explore the role of interleukin-6 （IL-6） in cervical cancer cell C-33A. Methods The cervical cancer cells C-33A were divided into the IL-6 group and the control group after culture. The IL-6 group were treated with 50 ng/mL of recombinant IL-6 protein, and the control group were without IL-6. Then cell viability and cell migration were detected by MTT assay and wound-healing assay, respectively. The mRNA and protein expressions of epithelial-cadherin （E-Cad）, neural-cadherin （N-Cad）, vimentin and transcription factors-snaill （TFs-SNAIL1） were analyzed by real time quantitative polymerase chain reaction and Western blotting, respectively. Results Compared with the control group, in the IL-6 group the proliferation of cervical cancer cells C-33A was promoted （12 h： 0.388±0.025 vs. 0.597±0.057; 24 h： 0.547±0.021 vs. 0.798±0.036; 48 h： 0.745±0.056 vs. 1.296±0.122; 72 h： 1.074±0.053 vs. 1.805±0.113; P〈0.05）, and the relative migration ability of cervical cancer cell was promoted （12 h： 1.057±0.029 vs. 1.200±0.045; 24 h： 1.189±0.036 vs. 1.428±0.181; 48 h： 1.273±0.059 vs. 1.569±0.143; 72 h： 1.409±0.047 vs. 1.623±0.170; P〈0.05）; meanwhile, compared with the control group, in the IL-6 group, the expression of E-Cad mRNA （1.012±0.098 vs. 0.483±0.171, P〈0.01） and E-Cad protein （1.032±0.015 vs. 0.395±0.119; P〈0.01） decreased, the expression of N-Cad mRNA （1.054±0.106 vs. 1.465±0.230, P〈0.01） and N-Cad protein （1.040±0.043 vs. 1.605±0.128, P〈0.01） increased, the expression ofvimentin mRNA （1.050±0.083 vs. 1.340±0.099, P〈0.05） and vimentin protein （1.043±0.062 vs. 1.430±0.077, P〈0.05） increased, and the expression ofTFs-SNAIL1 mRNA （1.058±0.176 vs. 1.510±0.229, P〈0.01） and Fs-SNAILI protein （1.022±0.015 vs. 1.470±0.139, P〈0.01） increased. Conclusion IL-6 may promote the proliferation, migration, and epithelial-mesenchymal transition of cervical cancer cell C-33A.