两种旋毛虫丝氨酸蛋白酶抑制剂对巨噬细胞炎性细胞因子mRNA表达的影响

Effects of two kinds of serine protease inhibitors on the expression of inflammatory cytokines mRNA in macrophages

丝氨酸蛋白酶抑制剂在寄生虫入侵宿主的过程中发挥着关键作用,能够参与到入侵宿主、免疫逃避、炎症反应、凝血系统和细胞迁徙等过程。为了探讨两种丝氨酸蛋白酶抑制剂(Ts Ad SPI和Ts Ka SPI)对巨噬细胞所分泌炎性细胞因子的调节作用,应用实时荧光定量PCR技术对两种SPIs分别处理的小鼠巨噬细胞进行TNF-α、IL-1β、IL-6、IL-12、IL-10、TGF-βmRNA表达水平的检测。结果显示,Ts Ad SPI和Ts Ka SPI均可不同程度的抑制LPS活化的小鼠巨噬细胞促炎性细胞因子TNF-α、IL-1β、IL-6和IL-12 mRNA的表达水平。并且两种SPI均可不同程度的促进巨噬细胞抗炎性细胞因子IL-10和TGF-β的表达。表明这两种旋毛虫SPI可通过调节宿主巨噬细胞影响入侵时宿主的免疫应答。

Serine protease inhibitors（ SPI） play a key role in the process of host parasite invasion. SPIs have been found to be able to participate in some processes of infection,including invading host,immune evasion,inflammation,coagulation and cell migration. In order to investigate the regulation function of two serine protease inhibitors（ Ts Ad SPI and Ts Ka SPI） to the secretion of inflammatory cytokines in macrophages,we detected the expression levels of TNF-alpha,IL-1 beta,IL-6,IL-12,IL-10 and TGF-beta mRNAs in mouse macrophages using real-time fluorescent quantitative PCR. The results showed that,Ts Ka SPI and Ts Ad SPI both inhibited the mRNA expression levels of pro-inflammatory cytokines TNF-alpha,IL-1 beta,IL-6 and IL-12 at different scales in J774 A. 1 mouse macrophages activated by LPS. And two kinds of SPIs both promoted the expression of anti-inflammatory cytokines IL-10 and TGF-beta in J774 A. 1 macrophages of mice with different degrees. These results suggest that the two SPIs can regulate host immune response by regulating the function of host macrophages.