放射工作人员血清中8-羟基脱氧鸟苷水平研究

Serum 8-hydroxy-2-deoxyguanosine level of radiation workers in hospitals

目的 探讨长期低剂量电离辐射对医疗机构的放射工作人员血清中8-羟基脱氧鸟苷（8-OHdG）水平的影响。方法 首先按放射工种分层，随后采用随机数表法选取307名性别、年龄匹配的放射工作人员，删去缺少剂量资料的对象后，共230例纳入本研究，包括放射诊断75例、放射治疗60例、核医学41例和介入放射学54例共4组。采用酶联免疫吸附试验（ELISA）测定其血清中8-OHdG水平。结果 放射诊断、放射治疗、核医学和介入放射学4组放射工作人员之间血清8-OHdG浓度比较，差异有统计学意义（F=9.071，P 〈 0.05），其中以介入放射工作人员的血清8-OHdG浓度最高（t=-4.773、-3.011、-2.189，P 〈 0.05）；在不同年有效剂量组和工龄组放射工作人员的血清8-OHdG水平存在差异（F=7.659、3.058，P 〈 0.05），随着受照剂量和放射工龄的增加，放射工作人员血清8-OHdG水平呈上升趋势（r=0.300、0.142，P 〈 0.05）。结论 血清中8-OHdG水平可能是暴露于低剂量电离辐射放射工作人员的DNA氧化损伤的潜在生物标志。

Objective To explore the influence of long-term low-dose ionizing radiation on 8-hydroxy-2-deoxyguanosine（8-OHdG）level in the serum of radiation workers in hospitals. Methods 307 age-and sex-matched hospital radiation workers were recruited by stratified random sampling method. After deleting the subjects without dosage information,230 individuals were divided into four groups according to their job title[including diagnostic radiology（n=75）, radiotherapy（n=60）, nuclear medicine（n=41）and interventional radiology（n=54）]. Serum 8-OHdG level was measured by ELISA assay. Results According to the statistical analysis, there was significant difference in the serum 8-OHdG level among four groups （F=9.071, P 〈 0.05）, and the content of serum 8-OHdG was significantly higher in the interventional radiology group than that in the groups of diagnostic radiology, radiotherapy and nuclear medicine （t=-4.473, -3.011, -2.189, P 〈 0.05）. There were significant differences in serum 8-OHdG level among different dose groups and working period groups（F=7.659, 3.058, P 〈 0.05）. The serum 8-OHdG levels significantly increased along with exposure dose and working period（r=0.300, 0.142, P〈0.05）.Conclusions Serum 8-OHdG may be a potential biomarker of oxidative DNA damage in radiation workers exposed to low-dose ionizing radiation.