介入法建立大鼠冠状动脉微栓塞模型

Establishment of a model of coronary microembolization in rats by intervention

目的:探讨自体血栓颗粒经右颈总动脉介入法建立大鼠冠状动脉微栓塞模型。方法:36只雄性SD大鼠随机分为:模型24 h组(n=10)、模型30 d组(n=10)、假手术组(n=10)、空白组(n=6)。模型24 h组、模型30 d组用介入法将0.5 m L大鼠自身血栓颗粒注入其主动脉根部,制成冠状动脉微栓塞模型。模型24 h组和模型30 d组分别在造模术24 h后、30 d后采血并处死大鼠,假手术组和空白组在30 d后处死大鼠。摘取心脏,取左心室前壁、心尖部切片,经HE染色、马休黄猩红蓝染色、苏木素碱性复红苦味酸染色计数栓塞的冠状微动脉阳性数量、缺血心肌的面积。结果:HE染色显示模型24 h组[(47.23±6.20)%]、模型30 d组[(62.88±15.68)%]被栓塞的阳性微动脉数量明显高于假手术组[(6.75±5.23)%,P<0.008];马休黄猩红蓝染色显示模型24 h组[(46.20±10.41)%]、模型30 d组[(55.30±16.34)%]被栓塞的阳性微动脉数量明显高于假手术组[(8.13±8.80)%,P<0.008];苏木素碱性复红苦味酸染色显示模型24 h组[(2.46±1.41)%]、模型30 d组[(6.52±2.22)%]染色阳性缺血面积明显高于假手术组[(0.05±0.02)%,P<0.017]。结论:介入法将大鼠自身血栓颗粒注入其冠状动脉内可以建立稳定、可靠、经济的冠状动脉微栓塞模型。

Objective：To study the feasibility of establishing the coronary microembolization in rats with injecting autologous micro thrombus particles by intervention via right common carotid artery. Methods：The 36 rats were randomly divided into 4 groups：24 hours coronary microembolization of model group （n=10）,30 days coronary microembolization of model group （ n = 10）, sham group（n=10） and normal group（n=6）. The rats in the coronary mieroembolization model group received 0.5 mL suspension of micro thrombus particles into the root of aorta by intervention through the right common carotid artery. The sham group rats were injected the same volume of normal saline. The rats of 24 hours model group and 30 days model group were sacrificed on scheduled dates, respectively. The left ventricular anterior and heart apex were cut into the serial sections at the thickness of 5 ~m. HE staining and martius-sarlet-blue staining were used to observe the ingredients of the vascular embolus and the number of positive arteries embolization. Hematoxylin-basic-fuchsin-picric staining was used to study the area of myocardial ischemia. Results： The numbers of positive arteries embolization of the 24 h model group[（47.23±6.20）%],the 30 days model group[（62.88± 15.68）%] were significantly higher than that of the sham group[（6.75 ±5.23）%] in the HE staining（P〈0.008）. There was no significant difference between the sham group and normal group. The amounts of positive arteries embolization of the 24 hours model group [（46.20 ± 10.41）%] and the 30 days model group[（55.30 ±16.34）%]were significantly higher than that of the sham group[（8.13± 8.80）%] in the martius-sarlet-blue staining（P〈0.008）. The area of myocardial ischemia in the 24 h model group[（2.46± 1.41 ）%] and the 30 days model group[ （6.52 ± 2.22）%] were significantly higher than that of the sham group[（0.05 ± 0.02）%] in the hematoxylin-basic-fuchsin-picric staining（P〈0.017）. Conclusion ：The coronary micro-embolization model of the rat can be built success- fully and economically with intervention.