生物活性玻璃治疗牙本质过敏症的长效性体外研究

Long-term in vitro effectiveness of a bioglass desensitizing agent investigated using electrochemical impedance spectroscopy,atomic force microscopy and scanning electron microscopy

目的体外研究比较生物活性玻璃与其他两种脱敏剂治疗牙本质过敏症的长期效果。方法体外制备50例牙本质盘样本,随机分为5组(N=10)。组1、2、3、4分别用生物活性玻璃、冷酸灵、高露洁抗敏和蒸馏水每天两次处理2分钟;组5为对照组。随后将样本浸入咖啡和纯可乐中5分钟。每周使用电化学阻抗仪检测牙本质渗透性。采用双因素方差分析和post hoc检验进行统计学分析。采用原子力显微镜和扫描电镜观察样本表面微形态改变。结果三种脱敏剂在1个月内均降低牙本质的渗透性并不同程度封闭牙本质小管。Post hoc LSD分析表明生物活性玻璃与冷酸灵相比能显著降低牙本质的渗透性(P<0.05),与高露洁抗敏无统计学差异(P=0.324)。结论三种脱敏剂都能在1个月内不同程度封闭牙本质小管、降低渗透性,生物活性玻璃和高露洁抗敏效果最佳。此外,生物活性玻璃类脱敏剂能快速起效。

Objective To compare the long-term in vitro effectiveness of a newer desensitizing agent containing bioactive glass and two other commercial products employed for treating dentin hypersensitivity. Methods Fifty occlusal dentin disks from extracted sound human third molars were treated with 0.5 M ethylenediaminetetraacetic acid for 2 min then randomly divided into five groups（n =10）. Specimens were brushed for 2 min twice daily with Actimins Paste（Group1）, Lesening Super Desensitizing Toothpaste（Group2）, Colgate Sensitive Pro-Relief Desensitising Polishing Paste（Group3） and distilled water（Group4） at 9：00 am and at 5：00 pm. Specimens in Group5 had no brushings. All specimens were immersed sequentially for 5 min into coffee（pH=5.4） at 10：00 am and undiluted cola（pH=2.5） at 6：00 pm. Dentin permeability was measured weekly over one month using electrochemical impedance spectroscopy（EIS）.Data were analyzed using two-way repeated measures ANOVA and post hoc LSD tests. Dentin microstructures were observed at one month by scanning electron microscopy and more details were revealed in the atomic force microscopy images. Results The three desensitizing agents significantly reduced dentin permeability over one month. Post hoc LSD tests of the EIS values indicated that brushing with Actimins showed no significant difference from Colgate Sensitive（P=0.32）, and that both these agents had significantly less permeability than Lesening（P〈0.05）. Conclusion The three desensitizing agents were effective in occluding dentinal tubules and reducing permeability to varying extents over one month, with Actimins and Colgate Sensitive being the most effective. And, Actimins might be a faster-working desensitizing agent for clinical use.